Thermal tolerant mannanase from acidothermus cellulolyticus
AssigneeMidwest Research Institute
InventorsShi-You Ding, William S. Adney, Todd B. Vinzant, Michael E. Himmel
Life of this patent
Estimating the term…
Abstract
The invention provides a thermal tolerant mannanase that is a member of the glycoside hydrolase family. The invention further discloses this mannanase as ManA. ManA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified ManA. Methods of making ManA polypeptides, including fusions, variants, and derivatives, are also disclosed. Methods of using mannanase A, including for the processing of food and for use in food stuffs as bulking agents and the like, are also disclosed.
Claims67
A composition comprising a substantially purified mannanase A peptide, the mannanase A peptide comprising a catalytic domain GH5, a carbohydrate binding domain III, and a carbohydrate binding domain II.
A mannanase A peptide having a sequence of SEQ ID NO:
- 1.
An isolated polynucleotide molecule comprising a nucleic acid sequence having an about 90% sequence identity to the nucleic acid sequence encoding the amino acid sequence SEQ ID NO:2.
An isolated polynucleotide molecule comprising a nucleic acid sequence encoding a heterologous protein in frame with the polypeptide molecule of SEQ ID NO:2.
An isolated polypeptide molecule comprising:
- a) a sequence of SEQ ID NO:
- 3;
- b) a sequence of SEQ ID NO:
- 4;
- c) a sequence of SEQ ID NO:
- 5;
- d) a sequence of SEQ ID NO:
- 1;
- e) a sequence of SEQ ID NO:
- 2;
- or
- f) an amino acid sequence having at least 70% sequence identity with the amino acid sequence of a), b), c), or d).
A method for assessing the carbohydrate degradation activity of mannanase A comprising:
- analyzing a carbohydrate degradation in the presence of mannanase A and a carbohydrate degradation in the absence of mannanase A on a substrate;
- and
- comparing the carbohydrate degradation in the presence of mannanase A with the carbohydrate degradation in the absence of mannanase A.
A method for assessing the carbohydrate degradation activity of mannanase A in the presence of an agent of interest comprising:
- analyzing a carbohydrate degradation in the presence of mannanase A and a carbohydrate degradation in the presence of mannanase A and the agent of interest on a substrate exposed;
- and
- comparing the carbohydrate degradation in the mannanase A treated substrate with the carbohydrate degradation in the mannanase A treated substrate in the presence of the agent of interest.
Dependencies are inferred from the claim text.