Soluble GlcNAc phosphotransferase
AssigneeNovazyme Pharmaceuticals, Inc.
InventorsWilliam Canfield, Mariko Kudo
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Abstract
The present invention relates to a soluble GlcNAc phosphotransferase, a method of making the same and a method of phosphorylating with the same.
Claims55
A method of phosphorylating a protein comprising contacting said protein with a soluble GlcNAc-phosphotransferase;
- and producing a phosphorylated protein.
An isolated polypeptide comprising SEQ ID NO:
- 2.
An isolated polynucleotide comprising SEQ ID NO:
- 1.
An isolated polynucleotide, which hybridizes under stringent conditions to the isolated polynucleotide SEQ ID NO:
- 1 or the complement of SEQ ID NO:
- 1.
An GlcNAc-phosphotransferase comprising an α subunit, a β subunit and a site-specific proteolytic cleavage site interposed between said α and β subunits, wherein said site-specific proteolytic cleavage site is not endogenous to GlcNAc-phosphotransferase.
The GlcNAc-phosphotransferase of claim 30, wherein said α-subunit comprises amino acids 1-928 of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.