Process for preparing stabilized reaction mixtures which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures

A process for preparing a stabilized and totally or partially dried reaction mixture, said mixture comprising at least one enzyme, comprising:

• a) bringing into contact in a single container:
• i) an aqueous solution of a reaction mixture comprising at least one enzyme;
• and
• ii) an aqueous solution of a stabilizing mixture composed of:
• at least one protective agent against drying;
• at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups;
• and
• at least one inert polymer capable of generating a mesh structure preventing the mobility of the dried reagents;
• in order to obtain an aqueous solution comprising said reaction mixture together with said stabilizing mixture;
• and
• b) removing all or part of the water contained in said aqueous solution obtained in step a), until obtaining a totally or partially dried mixture comprising said enzyme and said stabilizing mixture having a moisture content which is equal to or less than 30%, in order to obtain a stabilized reaction mixture which is totally or partially dried, comprising at least one enzyme.