US 2003/0119035 A1Published application

Tryptase-like polypeptide ztryp1

AssigneeZymoGenetics, Inc.

InventorsScott R. Presnell, David W. Taft

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Abstract

The present invention relates to polynucleotide and polypeptide molecules for mouse ztryp1, a novel member of the serine protease family of proteins. The polynucleotides encoding mouse ztryp1 can be used to identify a human ortholog or to create a mouse model associated with human disease states. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.

Claims18

Dependent claims

An isolated polynucleotide encoding a serine protease polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of:

(a) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 44 (Val) to amino acid number 276 (Ile);
(b) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 43 (Val) to amino acid number 275 (Arg);
(c) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 24 (Leu) to amino acid number 276 (Ile);
(d) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 19 (Arg) to amino acid number 275 (Arg);
(e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 44 (Val) to amino acid number 314 (Leu);
(f) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 43 (Val) to amino acid number 312 (Leu);
(g) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 24 (Leu) to amino acid number 314 (Leu);
(h) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 19 (Arg) to amino acid number 312 (Leu);
(i) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 314 (Leu),
(j) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 1 (Met) to amino acid number 312 (Leu), and
(k) the amino acid sequence as shown in SEQ ID NO:24;
and
wherein the amino acid percent identity is determined using a FASTA program with ktup=1, gap opening penalty=10, gap extension penalty=1, and substitution matrix=BLOSUM62, with other parameters set as default.

10 more elements

A DNA construct encoding a fusion protein, the DNA construct comprising:

a first DNA segment encoding a polypeptide that is selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO:
2 from residue number 1 (Met), to residue number 23 (Ser);
(b) the amino acid sequence of SEQ ID NO:
15 from residue number 1 (Met), to residue number 18 (Ala);
(c) the amino acid sequence of SEQ ID NO:
2 from residue number 24 (Leu), to residue number 43 (Lys);
(d) the amino acid sequence of SEQ ID NO:
15 from residue number 19 (Arg), to residue number 42 (Arg);
(e) the amino acid sequence of SEQ ID NO:
2 from residue number 24 (Leu), to residue number 276 (Ile);
(f) the amino acid sequence of SEQ ID NO:
15 from residue number 19 (Arg), to residue number 275 (Arg);
(g) the amino acid sequence of SEQ ID NO:2 from residue number 24 (Leu), to residue number 314 (Leu);
(h) the amino acid sequence of SEQ ID NO:15 from residue number 19 (Arg), to residue number 312 (Leu);
(9) the amino acid sequence of SEQ ID NO:
2 from residue number 44 (Val), to residue number 276 (Ile);
(j) the amino acid sequence of SEQ ID NO:
15 from residue number 43 (Val), to residue number 275 (Arg);
(k) the amino acid sequence of SEQ ID NO:
2 from residue number 44 (Val), to residue number 314 (Leu);
(l) the amino acid sequence of SEQ ID NO:
15 from residue number 43 (Val), to residue number 312 (Leu);
(m) the amino acid sequence of SEQ ID NO:
2 from residue number 277 (Lys), to residue number 314 (Leu);
(n) the amino acid sequence of SEQ ID NO:15 from residue number 276 (Thr), to residue number 312 (Leu);
and
(o) the amino acid sequence of SEQ ID NO:24;
and
at least one other DNA segment encoding an additional polypeptide,
wherein the first and other DNA segments are connected in-frame;
and
encode the fusion protein.

30 more elements

An isolated polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of:

(a) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 44 (Val) to amino acid number 276 (Ile);
(b) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 43 (Val) to amino acid number 275 (Arg);
(c) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 24 (Leu) to amino acid number 276 (Ile);
(d) the amino acid sequence as shown in SEQ ID NO:
15 from amino acid number 19 (Arg) to amino acid number 275 (Arg);
(e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 44 (Val) to amino acid number 314 (Leu);
(f) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 43 (Val) to amino acid number 312 (Leu);
(g) the amino acid sequence as shown in SEQ ID NO:
2 from amino acid number 24 (Leu) to amino acid number 314 (Leu);
(h) the amino acid sequence as shown in SEQ ID NO:
15 from amino acid number 19 (Arg) to amino acid number 312 (Leu);
(i) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 314 (Leu),
(j) the amino acid sequence as shown in SEQ ID NO:15 from amino acid number 1 (Met) to amino acid number 312 (Leu), and
(k) the amino acid sequence as shown in SEQ ID NO:24;
and
wherein the amino acid percent identity is determined using a FASTA program with ktup=1, gap opening penalty=10, gap extension penalty=1, and substitution matrix=BLOSUM62, with other parameters set as default.

13 more elements

Dependencies are inferred from the claim text.

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Abstract