Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
AssigneeCaprotec Bioanalytics GmbH
InventorsHubert Koster, Suhaib Siddiqi, Daniel P. Little
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Abstract
Capture compounds and collections thereof and methods using the compounds for the analysis of biomolecules are provided. In particular, collections, compounds and methods are provided for analyzing complex protein mixtures, such as the proteome. The compounds are multifunctional reagents that provide for the separation and isolation of complex protein mixtures. Automated systems for performing the methods also provided.
Claims125
A collection of capture compounds, comprising:
- a plurality of capture compounds, comprising sets of capture compounds, wherein set of each capture compounds includes a moiety X that is selected to covalently bind to biomolecules or to bind with sufficiently high affinity so that the resulting complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis;
- a moiety Y that increases the selectivity of the binding by X such that the capture compound binds to fewer biomlecules when the selectivity moiety is present than in its absence;
- a moiety Q, such that each set contains a different Q, wherein Q permits separation of each set;
- and a moiety Z for presenting X, Y and Q.
A system for analysis of mixtures of biomolecules, comprising:
- a collection of capture compounds, comprising sets of capture compounds, wherein set of each capture compounds includes a moiety X, which is different in each set, that is selected to covalently bind to biomolecules or to bind with sufficiently high affinity so that complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis;
- a moiety Y that increases the selectivity of the binding such that the capture compound binds to fewer biomlecules when the selectivity moiety is present than in its absence;
- a computer programmed with instructions for controlling and directing analysis of biomolecules using the collections;
- a mass spectrometer;
- and
- software for analysis of data produced by the mass spectrometer.
A collection of capture compounds, comprising:
- a plurality of capture compounds, comprising sets of capture compounds, wherein set of each capture compounds includes a moiety X, which is different in each set, that is selected to covalently bind to biomolecules or to bind with sufficiently high affinity so that the resulting complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis;
- a moiety Y that increases the selectivity of the binding by X such that the capture compound binds to fewer biomlecules when the selectivity moiety is present than in its absence;
- and a moiety Z for presenting X and Y.
A capture compound, comprising a moiety Z that is a trivalent trityl for presenting functional moieties X, Y and Q, wherein moiety X is selected to covalently bind to biomolecules or to bind with sufficiently high affinity so that the resulting complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis;
- moiety Y increases the selectivity of the binding by X such that the capture compound binds to fewer biomlecules when Y is present than in its absence;
- and moiety Q permits separation of sets of capture compounds.
Dependencies are inferred from the claim text.