Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays

A method for detecting single nucleotide polymorphism, comprising extending a probe hybridized to a target by exactly one base by incorporating a compound selected from the group consisting of a dideoxynucleoside α-thio triphosphate and an acyclonucleoside α-thio triphosphate.

A polynucleotide, comprising at least one residue of the group consisting of an adenosine thiophosphate residue, a deoxyadenosine thiophosphate residue, a cytidine thiophosphate residue, a deoxycytidine thiophosphate residue, a guanosine thiophosphate residue, a deoxyguanosine thiophosphate residue, a thymidine thiophosphate residue, and an uridine thiophosphate residue, and at least one moiety bonded to said at least one residue, said moiety selected from the group consisting of a maleimide and an iodoacetamide.

A molecular probe, wherein said probe terminates in a moiety selected from the group consisting of a thio dideoxynucleotide and an thio acyclonucleotide.

A microarray, comprising at least one molecular probe, said probe terminating in a moiety selected from the group consisting of a thio dideoxynucleotide and a thio acyclonucleotide.

A nucleic acid, said nucleic acid comprising at least three residues of the group consisting of an adenosine thiophosphate residue, a deoxyadenosine thiophosphate residue, a cytidine thiophosphate residue, a deoxycytidine thiophosphate residue, a guanosine thiophosphate residue, a deoxyguanosine thiophosphate residue, a thymidine thiophosphate residue, and a uridine thiophosphate residue.

A nucleic acid, comprising cRNA having a thiophosphate nucleotide.

A cRNA comprising at least one residue selected from the group consisting of an adenosine thiophosphate residue, a cytidine thiophosphate residue, a guanosine thiophosphate residue, and an uridine thiophosphate residue.

An expression assay kit, comprising a labeling reagent, and a nucleotide reagent, said labeling reagent comprising a thioreactive compound, and said nucleotide reagent comprising a nucleoside α-thiotriphosphate.

A single nucleotide polymorphism assay kit, comprising a labeling reagent, and a nucleoside triphosphate, said labeling reagent comprising a thioreactive compound, and said nucleoside triphosphate comprising a compound selected from the group consisting of a dideoxynucleoside α-thiotriphosphate and an acyclonucleoside α-thiotriphosphate.

A method of labeling a nucleic acid that terminates in a residue selected from the group consisting of a dideoxyadenosine thiophosphate residue, a dideoxyguanosine thiophosphate residue, a dideoxycytidine thiophosphate residue, a 3′-deoxythymine thiophosphate residue, and a dideoxyuridine thiophosphate residue, comprising reacting said nucleic acid with a thioreactive compound.

A method of labeling a nucleic acid that terminates in a residue selected from the group consisting of an acycloadenosine thiophosphate residue, an acycloguanosine thiophosphate residue, an acyclocytidine thiophosphate residue, a 3′-acyclothymine thiophosphate residue, and an acyclouridine thiophosphate residue, comprising reacting said nucleic acid with a thioreactive compound.