Nucleic acid which is upregulated in human tumor cells, a protein encoded thereby and a process for tumor diagnosis
OwnerSepp Kaul, Josef Preiherr +4inventors
InventorsSepp Kaul, Josef Preiherr, Erika Karolina Preiherr, Josef Preiherr, Ingrid Preiherr, Ulrich Weidle
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Abstract
A nucleic acid molecule (PKW) with the nucleic acid sequence SEQ ID NO:1 is upregulated in mammary carcinoma cells. The PKW protein of SEQ ID NO:2 and SEQ ID NO:4 is also provided. A process for determining whether a sample contains tumor cells is provided.
Claims2
A process for determining whether or not a test sample originating from or containing human cells has a tumor progression potential, wherein a second sample originating from non-tumor cells from the same individual or a different individual of the same species is also used, which process comprises the following steps:
- (a) incubating said samples under stringent hybridization conditions with a nucleic acid probe which is selected from the group consisting of:
- (i) a nucleic acid with a sequence of SEQ ID NO:1 or a fragment thereof;
- (ii) a nucleic acid with a sequence which is complementary to any nucleic acid of (i);
- (iii) a nucleic acid with a sequence which hybridizes under stringent conditions with the nucleic acid of (i);
- and
- (iv) a nucleic acid with a sequence which hybridizes under stringent conditions with the nucleic acid of (ii);
- and
- (b) determining the approximate amount of hybridization of each respective sample with said probe and
- (c) comparing the approximate amount of hybridization of the test sample to an approximate amount of hybridization of said second sample to identify whether or not the test sample contains a lower amount of the nucleic acid than does said second sample.
A process for determining whether or not a test sample originating from or containing human cells has a tumor progression potential, which process comprises the following steps:
- (a) incubating a first compartment of said sample under stringent hybridization conditions with a first nucleic acid probe which is selected from the group consisting of:
- (i) a nucleic acid with a sequence of SEQ ID NO:1 or a fragment thereof;
- (ii) a nucleic acid with a sequence which is complementary to any nucleic acid of (i);
- (iii) a nucleic acid with a sequence which hybridizes under stringent conditions with the nucleic acid of (i);
- and
- (iv) a nucleic acid with a sequence which hybridizes under stringent conditions with the nucleic acid of (ii);
- and
- (b) incubating a second compartment of said sample under stringent hybridization conditions with a second nucleic acid probe being a housekeeping gene or a fragment thereof;
- (c) determining the approximate amount of hybridization of said sample with said first and second probe;
- (d) identifying whether or not the test sample contains an at least 3-fold amount of nucleic acid hybridizing with the first probe in comparison to the amount of nucleic acid hybridizing with the second probe.
Dependencies are inferred from the claim text.