Novel estrogen-regulated G protein gamma subunit: compositions and methods of use
AssigneeSchering Corporation
InventorsAlan R. Brooks, Gary G. Deng, Gabor M. Rubanyi
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Abstract
The present invention provides novel nucleic acid and protein sequences for an estrogen-regulated GTP-binding protein gamma-12 subunit protein, as well as methods for using the sequences for modulating the effects of estrogen in mammalian cells, methods of detecting estrogen signaling in cells, and methods of identifying compounds capable of acting as an estrogen receptor agonist or antagonist.
Claims29
An isolated nucleic acid encoding an estrogen-regulated GTP-binding protein gamma-12 subunit protein, wherein the protein comprises the amino acid sequence of SEQ ID NO:
- 1.
An isolated estrogen-regulated GTP-binding protein gamma-12 subunit protein, wherein the protein comprises the amino acid sequence of SEQ ID NO:
- 1.
A method of modulating estrogen signaling in a mammalian cell, the method comprising modulating the level of expression or activity of an estrogen-regulated GTP-binding protein gamma-12 subunit protein.
A method of detecting the presence of estrogen signaling in a mammalian cell, the method comprising detecting the expression of a nucleic acid encoding an estrogen-regulated GTP-binding protein gamma-12 subunit protein.
A method of identifying a compound capable of acting as an estrogen receptor agonist or antagonist, the method comprising:
- (1) contacting a cell comprising an estrogen receptor with said compound;
- and
- (2) determining the functional effect of said compound on said cell, wherein an increase in the level of estrogen-regulated GTP-binding protein gamma-12 subunit mRNA, protein or protein activity in said cell indicates that said compound is capable of acting as an estrogen receptor agonist, and wherein a decrease in the level of estrogen-regulated GTP-binding protein gamma-12 subunit mRNA, protein or protein activity in said cell indicates that said compound is capable of acting as an estrogen receptor antagonist.
Dependencies are inferred from the claim text.