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US 2003/0118989 A1Published application
Differential isolation of cellular content for biological assays
OwnerCarsten Rosenow, Rini Mukherjee Saxenainventors
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Abstract
In one embodiment, methods are provided for differential isolation of cellular contents from different cells for further analysis. In one exemplary embodiment, the nucleic acids of bacteria are isolated from a mixture of bacteria and animal cells by lysing animal cells.
Claims66
A method for sample preparation comprising:
- obtaining a sample comprising a first type of cells and a second type of cells, wherein the first type of cells is at least twice as susceptible to a lysis agent as the second type of cells;
- and
- applying the lysis agent to break the first type of cells.
A method for detecting nucleic acids comprising
- obtaining a sample comprising a first type of cells and a second type of cells, wherein the first type of cells is at least twice as susceptible to a lysis agent as the second type of cells;
- applying the lysis agent to break the first type of cells;
- removing at least 60% of the second type of cells to obtain an isolate;
- preparing a nucleic acid sample from the isolate.
1 more element
A method for detecting nucleic acids comprising
- obtaining a sample comprising a first type of cells and a second type of cells, wherein the first type of cells is at least twice at susceptible to a lysis agent as the second type of cells;
- applying the lysis agent to break the first type of cells;
- removing at least 80% of the cellular content of first type of cells;
- lysing second type of cells to obtain an isolate;
- and
- preparing a nucleic acid sample from the isolate.
3 more elements
The method of claim 49 wherein the plurality of different nucleic acid probes comprises at least 100 probes.
Dependencies are inferred from the claim text.