Mouse monoclonal antibody (5-21-3) to human immunodeficiency virus gp41 protein

A murine derived hybridoma cell line ATCC HB 9628.

A monoclonal antibody produced by the hybridoma cell line ATCC 9628 designated the 5-21-3 monoclonal antibody.

A method for detecting a marker indicative of exposure to HIV I in a sample comprising forming an antibody/antigen complex between the epitope on HIV I gp41 formed by a first sequence of amino acids Ile-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn-Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-L eu-Leu-Glu-Leu-Asp-Lys with at least one flanking amino acid sequence of at least 5 amino acids in length either 3′ to the carboxy terminus or 5′ to the amino terminus of said first sequence, said flanking sequence having an amino acid sequence substantially corresponding to that found on native HIV I gp41 adjacent said first sequence and a antibody specific for that epitope, and detecting the presence or amount of the antibody/antigen complex formed.

An immunoassay for determining the presence or amount of antibody to HIV I gp41 in a test sample comprising incubating the test sample with a solid phase-bound binding material containing a target epitope having the immunological properties of the epitope on HIV I gp41 formed by a first sequence of amino acids Ile-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn-Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-L eu-Leu-Glu-Leu-Asp-Lys with at least one flanking amino acid sequence of at least 5 amino acids in length either 3′ to the carboxy terminus or 5′ to the amino terminus of said first sequence, said flanking sequence having an amino acid sequence substantially corresponding to that found on native HIV I gp41 adjacent said first sequence and with a probe antibody which specifically binds to the target epitope of the binding material, and then determining the presence or amount of the probe antibody bound or unbound to the binding material as an indication of the presence or amount of antibody to HIV I gp41 in the test sample.