Rapid screening procedure for inflammation mediators
OwnerRichard A. Cassidy, David G. Burleson +2inventors
InventorsRichard A. Cassidy, David G. Burleson, Marvin L. Salin, Angel V. Delgado
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Abstract
The invention is compositions and methods for determining the presence of one or more immune response mediators, comprising obtaining a solution containing polymorphonuclear neutrophils, exposing the neutrophils to a chromophore, allowing the chromophore to oxidize to form a luminescent compound, and measuring the level of visible or ultraviolet radiation.
Claims20
A method for determining the presence of one or more immune response mediators, comprising obtaining a solution containing polymorphonuclear neutrophils, exposing the neutrophils to a chromophore, allowing the chromophore to oxidize to form a luminescent compound, and measuring the level of visible or ultraviolet radiation.
The method of claim 8 wherein the groups include —CH═CH—, —N═N—, or —C(═O)—.
A method of treatment comprising determining the level of inflammation mediators present in a body fluid, and administering an agent counter to the inflammation mediator.
A method of treatment comprising the use of hemoglobin-conjugated fluorescein to evaluate endocytic processes that are indicators of inflammation mediation.
A method for screening or evaluating drugs that modulate one or more steps in the inflammation cascade comprising obtaining a solution containing polymorphonuclear neutrophils, exposing the neutrophils to a chromophore, allowing the chromophore to oxidize to form a luminescent compound, and measuring the level of visible or ultraviolet radiation.
A method of screening or evaluating various concentrations of drugs comprising obtaining a solution containing polymorphonuclear neutrophils, exposing the neutrophils to a chromophore, allowing the chromophore to oxidize to form a luminescent compound, and measuring the level of visible or ultraviolet radiation.
A method of screening or evaluating combination of drugs and/or combinations of concentrations comprising obtaining a solution containing polymorphonuclear neutrophils, exposing the neutrophils to a chromophore, allowing the chromophore to oxidize to form a luminescent compound, and measuring the level of visible or ultraviolet radiation.
Dependencies are inferred from the claim text.