Method of preserving tissue equivalent and tissue equivalent preserved in frozen state
OwnerNaoka Yamamoto, Masayo Nomura +1inventors
InventorsNaoka Yamamoto, Masayo Nomura, Takeshi Moriyama
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Abstract
A method for cryopreservation of a tissue equivalent whereby the viability of frozen cells and the biological activity of thawed cells are improved and the steps are simplified; and a cryopreserved tissue equivalent obtained by the method. Cells suspended in a cryopreserving solution are inoculated into a matrix and then frozen before the cells adhere to the matrix.
Claims11
A method for cryopreservation of a tissue equivalent which comprises a step of suspending cells into a cryopreserving solution, a step of inoculating the cells on a matrix, and a step of freezing the thus obtained tissue equivalent before the cells adhere to the matrix.
A cryopreserved tissue equivalent obtained by inoculating cells suspended in a cryopreserving solution on a matrix and freezing it before the cells adhere to the matrix.
Dependencies are inferred from the claim text.