Compositions, preparations and uses of human papillomavirus L1 protein
OwnerStephen C. Harrison, Xiaojiang Cheninventors
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Abstract
Large quantities of soluble multimers of human papillomavirus L1 proteins can be produced in bacterial expression systems and used as therapeutic and diagnostic tools. L1 multimers can be used in immunogenic vaccine compositions, as diagnostic reagents, and as tools for mapping cell surface receptor interactions. The invention was made using U.S. Government funds, and therefore the U.S. Government has rights in the invention.
Claims45
A method of immunizing a human against a human papillomavirus, comprising the step of:
- administering to a human a composition which comprises a multimer of a human papillomavirus L1 protein, wherein the L1 protein is truncated at its amino terminus, at a dose effective to induce an immune response against the L1 protein in the human.
A method of detecting the presence of antibodies against a human papillomavirus in a biological sample, comprising the steps of:
- contacting a biological sample with a multimer of a human papillomavirus L1 protein, wherein the L1 protein is truncated at its amino terminus;
- and
- detecting antibodies which bind to the multimer, wherein detection of antibodies which are bound to the multimer identifies the presence of antibodies against the human papillomavirus in the biological sample.
A method of detecting a specific subtype of human papillomavirus in a biological sample, comprising the steps of:
- contacting a biological sample with an antibody which specifically binds to an L1 protein of a specific subtype of human papillomavirus, wherein the L1 protein is truncated at its amino terminus;
- and
- detecting L1 protein which is bound to the antibody, wherein detection of L1 protein which is bound to the antibody identifies the presence of the specific subtype of human papillomavirus in the biological sample.
A solid support comprising a multimer of a human papillomavirus L1 protein, wherein the L1 protein is truncated at its amino terminus.
A solid support comprising an antibody which specifically binds to an L1 protein of a specific human papillomavirus.
A method of producing a soluble multimer of a human papillomavirus L1 protein, comprising the steps of:
- expressing a recombinant human papillomavirus L1 protein in a bacterial host cell, wherein the recombinant human papillomavirus L1 protein comprises amino acids 13-505 as shown in SEQ ID NO:1;
- and
- treating the recombinant human papillomavirus L1 protein to remove bacterial host cell proteins, whereby a soluble multimer of the human papillomavirus L1 protein is formed.
A method of producing an T=1 icosahedral particle which comprises twelve pentamers of a human papillomavirus 16 L1 protein, comprising the steps of:
- expressing a recombinant human papillomavirus 16 L1 protein in an E. coli cell, wherein the recombinant L1 protein is truncated by 9, 10, 11, or 12 consecutive amino acids at its amino terminus;
- treating the recombinant L1 protein to remove GroEL protein, whereby soluble pentamers of L1 protein are formed;
- and
- placing the soluble pentamers at pH 4-5 at a concentration of at least 0.2 mg/ml, whereby T=1 icosahedral particles comprising twelve L1 pentamers are formed.
Dependencies are inferred from the claim text.