Methods for treating ischemic reperfusion injury using IkappaB kinase-beta inhibitors
OwnerAnthony Rosenzweig, Youngkeun Ahninventors
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Abstract
The present invention relates to methods and compositions for reducing or preventing ischemia-reperfusion injury. Methods for identifying candidate compounds for such treatment are also described.
Claims33
A method for reducing or preventing ischemic reperfusion injury to an organ in a mammal, said method comprising administering to said organ an IKK-β inhibitor in an amount sufficient to reduce or prevent said ischemic reperfusion injury.
A method for identifying a candidate compound for reducing or preventing ischemic reperfusion injury, said method comprising:
- (a) contacting a cell expressing an IKK-β gene with a candidate compound;
- and
- (b) measuring IKK-β gene expression or IKK-β protein activity in said cell, wherein a candidate compound that reduces said expression or said activity, relative to IKK-β expression or activity in a cell not contacted with said candidate compound, is a candidate compound useful for reducing or preventing ischemic reperfusion injury.
A method for identifying a candidate compound for reducing or preventing ischemic reperfusion injury, said method comprising:
- (a) contacting IKK-β protein with a candidate compound;
- and
- (b) determining whether said candidate compound binds said IKK-β protein, wherein a candidate compound that binds said IKK-β protein is a candidate compound useful for reducing or preventing ischemic reperfusion injury.
A kit comprising:
- (a) a vector expressing a nucleic acid encoding a dominant negative IKK-β protein;
- and
- (b) instructions for delivery of said vector to an organ for reducing or preventing ischemic reperfusion injury.
A vector comprising a polynucleotide encoding a dominant negative IKK-β protein operably linked to an organ-specific promoter.
Dependencies are inferred from the claim text.