Modified enzymes
AssigneeNovozymes A/S
InventorsHeinz Josef Deussen, Claus Crone Fuglsang, Arne Agerlin Olsen
Life of this patent
Estimating the term…
Abstract
The present invention relates to modified enzymes comprising an enzyme and at least one polyanionic domain, e.g. polyglutamic acid, polyaspartic acid or a polycarboxylic acid, wherein the enzyme comprises or is covalently attached to each said polyanionic domain. The present invention also relates to oral care compositions comprising such modified enzymes and use of the oral care compositions for preventing or treating dental disease, in particular for preventing or removing plaque. The modified enzymes are able to bind to hydroxylapatite in teeth.
Claims33
A modified enzyme comprising an enzyme and at least one polyanionic domain, wherein the enzyme comprises or is covalently attached to each said polyanionic domain.
The modified enzyme of any of the preceding claims, wherein said polyanionic domain is covalently attached to a carboxylate group and/or an amino group of the enzyme.
The modified enzyme of any of the preceding claims, wherein the polyanionic domain is covalently attached to the enzyme by means of at least one C—N bond, the carbon atom originating from the enzyme and the nitrogen atom originating from the polyanionic domain.
The modified enzyme of any of the preceding claims, wherein said enzyme is selected from the group consisting of oxidoreductases, proteases, lipases, glucanases, esterases, deaminases, ureases and polysaccharide hydrolases.
The modified enzyme of any of the preceding claims, wherein the catalytic activity of said modified enzyme is at least 1% of the catalytic activity of the free enzyme, preferably at least 2%, such as at least 5%, e.g. at least 10%, more preferably at least 20%, such as at least 30%, e.g. at least 40%, still more preferably at least 50%, such as at least 60%, e.g. at least 70%, even more preferably at least 80%, such as at least 90%, e.g. at least 95%, most preferably the modified enzyme is substantially identical to the catalytic activity of the free enzyme, as determined according to “Methods of Enzymatic Analysis”, 3rd. Edition, vol. 1-10, 1984, Verlag Chemie, Weinheim.
The modified enzyme of any of the proceeding claims, wherein said modified enzyme is capable of binding to hydroxylapatite (HAP), fluoroapatite, calcium phosphate, teeth or bone.
An oxidoreductase modified by covalent bonding to one or more polyglutamate and/or polyaspartate anions.
Dependencies are inferred from the claim text.