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AssigneeBoard of Trustees of the University of Arkansas
InventorMark D. Crew
Estimating the term…
The invention comprises exploiting viral stealth mechanisms to eliminate pig MHC class I cell-surface expression. PK(15) (pig kidney) cells stably transfected with the Herpes Simplex Virus (HSV) ICP47 gene [PK(15)-ICP47 cells] exhibited a dramatic reduction of MHC class I cell-surface expression when compared to untransfected PK(15) cells. To test the effect of down-regulation of porcine MHC class I on human cellular immune responses, a human CD8+ enriched T cell line (anti-PK15 T cells) with reactivity towards PK(15) cells was derived by repeated stimulation of human T cells with PK(15) cells stably transfected with the co-stimulatory molecule B7.1 [PK(15)-B7.1 cells]. Anti-PK15 T cells efficiently lysed PK(15) cells but not PK(15)-ICP47 (class I negative) cells. Consistent with effector function, anti-PK15 T cells showed a robust proliferative response to PK(15)-B7.1 cells but did not proliferate at all to PK(15)-B7.1 cells which also expressed HSV ICP47.
An isolated or synthetic protein comprising an amino acid sequence selected from the group consisting of:
An isolated nucleic acid molecule having a nucleotide sequence selected from the group consisting of:
A method of generating xenoreactive human T cells, comprising introducing into a nonhuman cell, a sequence selected from the group consisting of:
A method of inhibiting recognition of a nonhuman cell after xenotransplantation, comprising introducing into said nonhuman cell, a sequence selected from the group consisting of:
A method of conferring resistance of a nonhuman cell after xenotransplantation, comprising introducing into said nonhuman cell, a sequence selected from the group consisting of:
A method of inhibiting human T cell proliferative response to a nonhuman cell, after xenotransplantation, comprising introducing into said nonhuman cell, a sequence selected from the group consisting of:
Dependencies are inferred from the claim text.