Compositions and methods for isolation, propagation, and differentiation of human stem cells and uses thereof
AssigneeLevesque Biosciences, Inc.
InventorsToomas Neuman, Michel Levesque
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Abstract
The invention is directed to the field of human stem cells and includes methods and compositions for isolating, propagating, and differentiating human stem cells. The invention provides therapeutic uses of the methods and compositions, including autologous transplantation of treated cells into humans for treatment of Parkinson's and other neuronal disorders.
Claims33
A method for treating a patient suffering from a central nervous system disorder comprising:
- (a) obtaining central nervous system (CNS) stem cells;
- (b) differentiating the CNS stem cells by culturing them in vitro in differentiation medium comprising fibroblast growth factor 8 (FGF8) and glial cell line derived neurotrophic factor (GDNF);
- and
- (c) transplanting the differentiated CNS stem cells into the central nervous system of the patient.
A method of treating a patient suffering from Parkinson's disease comprising:
- (a) obtaining central nervous system (CNS) stem cells from the patient;
- (b) proliferating the CNS stem cells by culturing the cells in a first medium comprising bFGF, LIF and EGF;
- (c) differentiating the CNS stem cells by culturing the cells in a second medium comprising RA, dBcAMP, GDNF and FGF8;
- and
- (d) transplanting the differentiated cells into the patient's brain.
A cell culture medium for stimulating the proliferation of central nervous system (CNS) stem cells comprising about 0.2 to about 200 ng/ml each of basic fibroblast growth factor (bFGF), leukemia inhibitor factor (LIF), and epidermal growth factor (EGF).
A cell culture medium for differentiating central nervous system (CNS) stem cells comprising about 10−8 to about 10−4 M RA, about 0.01 to about 3 mM dBcAMP, about 0.02 to about 200 ng/ml FGF8, and about 0.02 to about 200 ng/ml GDNF.
Dependencies are inferred from the claim text.