Vector constructs for gene-therapy mediated radionuclide therapy of undifferentiated and medullary thyroid carcinomas and non-thyroidal tumours and metastases mediated thereof
OwnerThorsten Petrich, Eyck Potterinventors
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Abstract
The invention relates to vector constructs, comprising vector DNA which includes regulatory sequences, the NIS gene coding for the sodium/iodide symporter, the TPO gene coding for thyroidal peroxidase and use thereof for production of a medicament/diagnostic for treatment/diagnosis of tumour disease states, whereby treatment occurs before or concurrent with a radionuclide therapy, in particular, with iodine-131, or astatine-211. The invention further relates to use of two or several vector constructs for production of a medicament/diagnostic for treatment/diagnosis of tumour disease states.
Claims19
Vector construct, comprising vector DNA including regulatory sequences as well as the NIS gene encoding the sodium/iodide symporter and the TPO gene encoding the thyroid peroxidase.
The vector construct of any of the preceding claims, characterized in that it comprises the SV40-PE, CMV, or a tissue-specific promoter.
The vector construct of any of the preceding claims, characterized in that it further comprises an origin of replication and/or a marker gene, e.g., an antibiotic resistance gene, and/or a polyadenylation signal.
The vector construct of any of the preceding claims, further comprising a multiple cloning site (MCS), preferably in a position in 5′ direction of the NIS gene.
The vector construct of any of the preceding claims, characterized in that it is included in a liposome, wherein the liposomes may exhibit membrane-bound antibodies, in particular monoclonal antibodies, or other proteins, in particular receptor ligands, specific for tumor surface structures.
Vector construct of any of the preceding claims for the use in human or veterinary medicine.
Use of two or more vector constructs for the manufacture of a medicament/diagnostic agent for the treatment/diagnosis of tumor diseases, wherein the treatment is carried out prior to or simultaneously with a radionuclide therapy, in particular with iodine-131 or astate-211, characterized in that the two or more vector constructs each contain vector DNA including regulatory sequences and, in different constructs, the NIS gene encoding the sodium/iodide symporter, the TPO gene encoding the thyroid peroxidase, and optionally the TG gene encoding the human thyreoglobulin or any of its sub-units, wherein the two or more vector constructs are optionally included in liposomes, wherein the liposomes may exhibit membrane-bound antibodies, in particular monoclonal antibodies, or other proteins, in particular receptor ligands, specific for tumor surface structures, or are included in adenoviral, retroviral, or other viral vectors of human or animal pathogenicity, wherein the construct may exhibit on its surface natural or artificial tissue-specific, membrane-bound proteins (e.g., fiber proteins), or receptor ligands specific for tumor surface structures, or are included in both
Dependencies are inferred from the claim text.