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AssigneeTrustees of Tufts College
InventorsKim Lewis, Peichung Hsieh
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The present invention relates to the enhanced cytoplasmic accumulation of amphipathic weakly basic or amphipathic cationic molecules in prokaryotes and eukaryotes under conditions of high extracellular pH. Furthermore, the present invention relates to the unexpected synergistic effects of high extracellular pH and disrupted cellular efflux mechanisms on the cytoplasmic accumulation of amphipathic weakly basic or amphipathic cationic molecules in prokaryotes and eukaryotes. The present invention relates to methods for increasing the therapeutic potency of amphipathic weakly basic or amphipathic cationic compounds, e.g. antiseptics and disinfectants. Finally, the present invention relates to the exploitation of the aforementioned discoveries in the screening of small molecules, and libraries thereof, for biological activity in prokaryotes and eukaryotes.
A method for increasing the accumulation in a cell of amphipathic weak bases, amphipathic cations, or both, comprising culturing said cell in a medium with pH of about 9.
A formulation comprising amphipathic weakly basic molecules, amphipathic cationic molecules, or both;
A method for increasing the accumulation in a cell of amphipathic weak acids, amphipathic anions, or both, comprising culturing said cell in a medium with pH of about 5.
A formulation comprising amphipathic weakly acidic molecules, amphipathic anionic molecules, or both;
Dependencies are inferred from the claim text.