Multi-color multiplexed analysis in a bio-separation system

A bio-separation instrument, comprising:

• a separation channel;
• means for separating samples in the separation channel into analytes;
• and
• a detection system, comprising:
• a detection section along each separation channel defining a detection zone for analytes;
• a plurality of radiation sources, at least two of the radiation sources have different wavelength;
• excitation means for introducing excitation radiations from the radiation sources at the detection zone as analytes pass the detection zone;
• detecting means for detecting radiation emissions;
• control means for controlling the radiation sources and detecting means in a manner such that excitation radiations from the excitation sources are introduced at the detection zone in a predetermined sequence and radiation emission from the detection zone associated with each radiation source is detected in a time staggered/multiplexed manner.

A method for detecting analytes in a bio-separation device having a separation channel in which bio-separation takes place, comprising the steps of:

• defining a detection zone for analytes along the separation channel;
• providing a plurality of radiation sources, at least two of which associated with different wavelengths;
• introducing excitation radiations from that plurality of radiation sources at the detection zone as analytes pass the detection zone;
• providing a detector for detecting radiation emissions;
• controlling the radiation sources and detector in a manner such that excitation radiations from the radiation sources are introduced at the detection zone in a predetermined sequence and radiation emission from the detection zone associated with each radiation source is detected in a time staggered/multiplexed manner.