Genetically engineered duckweed
AssigneeNorth Carolina State University
InventorsAnne-Marie Stomp, Nirmala Rajbhandari
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Abstract
Methods and compositions for the efficient transformation of duckweed are provided. Preferably, the methods involve transformation by either ballistic bombardment or Agrobacterium. In this manner, any gene or nucleic acid of interest can be introduced and expressed in duckweed plants. Transformed duckweed plants, cells, tissues are also provided. Transformed duckweed plant tissue culture and methods of producing recombinant proteins and peptides from transformed duckweed plants are also disclosed.
Claims60
A method for transforming duckweed with a nucleotide sequence of interest, wherein the nucleotide sequence comprises at least one expression cassette comprising a gene which confers resistance to a selection agent, the method comprising the steps of:
- (a) providing a duckweed tissue target, the cells of the duckweed tissue including cell walls;
- and
- (b) propelling the nucleotide sequence at the duckweed tissue target at a velocity sufficient to pierce the cell walls and deposit the nucleotide sequence within a cell of the tissue to thereby produce a transformed tissue,
- wherein the nucleotide sequence is carried by a microprojectile;
- and
- wherein the nucleotide sequence is propelled at the tissue by propelling the microprojectile at the tissue.
A method for transforming duckweed with a nucleotide sequence of interest, the method comprising the steps of:
- (a) inoculating a duckweed plant tissue with an Agrobacterium comprising a vector which comprises the nucleotide sequence, wherein the nucleotide sequence comprises at least one expression cassette comprising a gene which confers resistance to a selection agent;
- and
- (b) co-cultivating the tissue with the Agrobacterium to produce transformed tissue.
A method for transforming a duckweed cell with a nucleotide sequence of interest, wherein the nucleotide sequence comprises at least one expression cassette comprising a gene which confers resistance to a selection agent, the method comprising the step of introducing the nucleotide sequence into a duckweed cell by electroporation.
A transformed duckweed plant comprising a heterologous nucleic acid of interest incorporated in its genome.
A method of producing recombinant proteins or peptides, comprising the steps of
- (a) culturing a transformed duckweed plant that expresses at least one heterologous protein or peptide;
- and
- (b) collecting the at least one protein or peptide from the duckweed cultures.
Dependencies are inferred from the claim text.