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AssigneeAjinomoto Co., Inc.
InventorsKatsura Izui, Tetsuya Miwa, Hiroaki Kisaka, Ai Akiyama
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The present invention provides a transformed plant having free-amino acid content increased by introducing phosphoenolpyruvate carboxylase (PEPC) genes, and also a progeny thereof and a seed thereof. Namely, the transformed plant has a nucleic acid construct containing PEPC gene introduced therein, wherein phosphoenolpyruvate carboxylase encoded by the PEPC gene does not require phosphorylation for the activation thereof and/or is independent of acetyl CoA. In particular, the present invention provides a transformed plant comprising a nucleic acid construct containing a nucleic acid molecule encoding Synechococcus vulcanus PEPC or a nucleic acid construct containing a nucleic acid molecule encoding a protein having PEPC activity which has at least 80% of nucleotide sequence homology to said gene.
A transformed plant containing phosphoenolpyruvate carboxylase gene introduced therein, wherein said phosphoenolpyruvate carboxylase encoded by the gene does not require phosphorylation for the activation thereof and/or said phosphoenolpyruvate carboxylase is independent of acetyl CoA for its activity.
A transformed plant containing phosphoenolpyruvate carboxylase gene introduced therein, wherein said phosphoenolpyruvate carboxylase encoded by the gene does not require phosphorylation for the activation thereof and said phosphoenolpyruvate carboxylase is independent of acetyl CoA for its activity.
A transformed plant having phosphoenolpyruvate carboxylase gene introduced therein, wherein said phosphoenolpyruvate carboxylase gene is derived from cyanobacteria.
A transformed plant containing a nucleic acid construct containing a nucleic acid molecule having at least 80% nucleotide sequence homology to Synechococcus vulcanus PEPC gene.
A method for producing a transformed plant having free-amino acids content higher than that of an untransformed plant of the same type cultivated under the same condition, which comprises the step of introducing a nucleic acid construct containing phosphoenolpyruvate carboxylase gene into a plant, wherein phosphoenolpyruvate carboxylase encoded by the gene does not require phosphorylation for the activation thereof and/or said phosphoenolpyruvate carboxylase is independent of acetyl CoA for its activity.
A method for producing a transformed plant having free-amino acids content higher than that of an untransformed plant of the same type cultivated under the same condition, which comprises the step of introducing a nucleic acid construct containing phosphoenolpyruvate carboxylase gene into a plant, wherein phosphoenolpyruvate carboxylase encoded by the gene does not require phosphorylation for the activation thereof and said phosphoenolpyruvate carboxylase is independent of acetyl CoA for its activity.
A method for producing a transformed plant having a free amino acids content higher than that of a natural plant of the same kind cultivated under the same condition, which comprises the step of introducing a nucleic acid construct containing phosphoenolpyruvate carboxylase gene into a plant, wherein said phosphoenolpyruvate carboxylase gene is derived from cyanobacterium.
A method for producing a transformed plant having a free-amino acids content higher than that of an untransformed plant of the same type cultivated under the same condition, which comprises the step of introducing a nucleic acid construct containing a nucleic acid molecule having at least 80% nucleotide sequence homology to Synechococcus vulcanus PEPC gene.
Dependencies are inferred from the claim text.