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US 2003/0115635 A1Published application
DNA encoding a plant deoxyhypusine synthase, a plant eukaryotic initiation factor 5A, transgenic plants and a method for controlling senescence programmed and cell death in plants
AssigneeSenesco, Inc.
InventorsJohn E. Thompson, Tzann-Wei Wang, Dongen Lily Lu
Life of this patent
Estimating the term…
Abstract
Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced eIF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced eIF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.
Claims60
An isolated DNA molecule encoding senescence-induced deoxyhypusine synthase, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 1 and/or SEQ ID NO:
- 9, or a functional derivative of the isolated DNA molecule which hybridizes with SEQ ID NO:
- 1 and/or SEQ ID NO:
- 9 with the proviso that the DNA molecule does not have the sequence of SEQ ID NO:
- 5.
2 more elements
An isolated senescence-induced deoxyhypusine synthase encoded by a nucleotide sequence which hybridizes under low stringency conditions with SEQ ID NO:
- 1and/or SEQ ID NO:
- 9, or a functional derivative of the senescence-induced deoxyhypusine synthase.
An isolated DNA molecule encoding senescence-induced eIF-5A, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 11, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15, or a functional derivative of the isolated DNA molecule which hybridizes with SEQ ID NO:
- 11, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15.
3 more elements
A vector for transformation of plant cells comprising
- (a) antisense nucleotide sequences substantially complementary to (1) a corresponding portion of one strand of a DNA molecule encoding senescence-induced deoxyhypusine synthase wherein the DNA molecule encoding senescence-induced deoxyhypusine synthase hybridizes under low stringency conditions with SEQ ID NO:
- 1, SEQ ID NO:
- 5 and/or SEQ ID NO:
- 9 or (2) a corresponding portion of an RNA sequence encoded by the DNA molecule encoding senescence-induced deoxyhypusine synthase;
- and
- (b) regulatory sequences operatively linked to the antisense nucleotide sequences such that the antisense nucleotide sequences are expressed in a plant cell into which it is transformed.
3 more elements
A vector for transformation of plant cells comprising (a) antisense nucleotide sequences substantially complementary to (1) a corresponding portion of one strand of a DNA molecule encoding senescence-induced eIF-5A wherein the DNA molecule encoding senescence-induced eIF-5A hybridizes under low stringency conditions with SEQ ID NO:
- 11, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15 or (2) a corresponding portion of an RNA sequence encoded by the DNA molecule encoding senescence-induced eIF-5A;
- and
- (b) regulatory sequences operatively linked to the nucleotide sequences such that the antisense nucleotide sequences are expressed in a plant cell into which it is transformed.
2 more elements
An antisense oligonucleotide or polynucleotide encoding an RNA molecule which is substantially complementary to (i) a corresponding portion of an RNA transcript of a plant senescence-induced deoxyhypusine synthase gene, wherein said plant gene hybridizes under low stringency conditions with SEQ ID NO:
- 1, SEQ ID NO:
- 5 and/or SEQ ID NO:
- 9 or (ii) a corresponding portion of an RNA transcript of a plant senescence-induced eIF-5A gene, wherein said plant gene hybridizes under low stringency conditions with SEQ ID NO:
- 11, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15.
3 more elements
A vector comprising a DNA molecule encoding
- (a) senescence-induced deoxyhypusine synthase, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 1, SEQ ID NO:
- 5, and/or SEQ ID NO:
- 9;
- and
- (b) regulatory sequences operatively linked to the DNA molecule such that the deoxyhypusine synthase is expressed in a plant cell into which it is transformed.
3 more elements
A vector comprising a DNA molecule encoding
- (a) senescence-induced eIF-5A, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 11, SEQ ID NO:
- 13, and/or SEQ ID NO:
- 15;
- and
- (b) regulatory sequences operatively linked to the DNA molecule such that eIF-5A is expressed in a plant cell into which it is transformed.
3 more elements
A vector comprising a DNA molecule encoding
- (a) senescence-induced deoxyhypusine synthase, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 1, SEQ ID NO:
- 5, and/or SEQ ID NO:
- 9;
- (b) senescence-induced eIF-5A, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 11, SEQ ID NO:
- 13, and/or SEQ ID NO:
- 15;
- and
- (b) regulatory sequences operatively linked to the DNA molecule such that the senescence-induced deoxhypusine synthase and the eIF-5A are expressed in a plant cell into which it is transformed.
7 more elements
A method for inhibiting the expression of endogenous senescence-induced deoxyhypusine synthase, eIF-5A or both in a plant, said method comprising
- (1) integrating into the genome of the plant a vector comprising
- (A) antisense nucleotide sequences substantially complementary to (i) a corresponding portion of one strand of a DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase, wherein the DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 1, SEQ ID NO:
- 5, and/or SEQ ID NO:
- 9 or (ii) a corresponding portion of an RNA sequence encoded by the endogenous senescence-induced deoxyhypusine synthase gene, (iii) a corresponding portion of one strand of a DNA molecule encoding the endogenous senescence-induced eIF-5A, wherein the DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 11, SEQ ID NO:
- 13, and/or SEQ ID NO:
- 15, (iv) a corresponding portion of an RNA sequence encoded by the endogenous senescence-induced eIF-5A, or (v) a combination of (I) or (ii) and (iii) or (iv);
- and
- (B) regulatory sequences operatively linked to the antisense nucleotide sequences such that the antisense nucleotide sequences are expressed;
- and
- (2) growing said plant, whereby said antisense nucleotide sequences are transcribed and bind to said RNA sequence, whereby expression of the senescence-induced deoxyhypusine synthase gene, senescence-induced eIF-5A gene or both is inhibited.
9 more elements
A method for inhibiting the expression of an endogenous senescence-induced deoxyhypusine synthase gene in a plant cell, said method comprising
- (1) integrating into the genome of at least one cell of the plant a vector comprising
- (A) an isolated DNA molecule encoding exogenous senescence-induced deoxyhypusine synthase, wherein the DNA molecule hybridizes under low stringency conditions with SEQ ID NO:
- 1, SEQ ID NO:
- 5, and/or SEQ ID NO:
- 9 or a functional derivative of the isolated DNA molecule which hybridizes with SEQ ID:
- 1, SEQ ID NO:
- 5, and/or SEQ ID NO:
- 9;
- and
- (B) regulatory sequences operatively linked to the DNA molecule such that the exogenous senescence-induced deoxyhypusine synthase encoded thereby is expressed;
- and
- (2) growing said plant, whereby said DNA molecule is over-expressed and the endogenous senescence-induced deoxyhypusine synthase gene is inhibited by exogenous senescence-induced deoxyhypusine synthase.
9 more elements
A method of altering age-related senescence and/or environmental stress-related senescence in a plant, said method comprising
- (1) integrating into the genome of the plant a vector comprising
- (A) antisense nucleotide sequences substantially complementary to (I) a corresponding portion of one strand of a DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase, wherein the DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 1, SEQ ID NO:
- 5 and/or SEQ ID NO:
- 9 or (ii) at least a portion of an RNA sequence encoded by the endogenous senescence-induced deoxyhypusine synthase gene, (iii) a corresponding portion of one strand of a DNA molecule encoding the endogenous senescence-induced eIF-5A gene, wherein the DNA molecule encoding the endogenous senescence-induced eIF-5A hybridizes with SEQ ID NO:
- 11, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15, (iv) a corresponding portion of an RNA sequence encoded by the endogenous senescence-induced eIF-5A gene, or (v) a combination of (I) or (ii) and (iii) or (iv);
- (B) regulatory sequences operatively linked to the antisense nucleotide sequences such that the antisense nucleotide sequences are expressed;
- and
- (2) growing said plant, whereby said antisense nucleotide sequences are transcribed and bind to said RNA sequence, whereby expression of said senescence-induced deoxyhypusine synthase gene, senescence-induced eIF-5A gene or both is inhibited.
8 more elements
A plant and progeny thereof, wherein said plant is derived from a cell having inhibited or reduced expression of senescence-induced deoxyhypusine synthase, senescence-induced eIF-5A, or both, wherein said cell is produced by
- (1) integrating into the genome of the cell a vector comprising
- (A) antisense nucleotide sequences substantially complementary to (i) a corresponding portion of one strand of a DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase, wherein the DNA molecule encoding the endogenous senescence-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 1, SEQ ID NO:
- 5, and/or SEQ ID NO:
- 9 or (ii) a corresponding portion of an RNA sequence encoded by the endogenous senescence-induced deoxyhypusine synthase gene, (iii) a corresponding portion of one strand of a DNA molecule encoding the endogenous senescence-induced eIF-5A gene, wherein the DNA molecule encoding the endogenous senescence-induced eIF-5A hybridizes with SEQ ID NO:l1, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15, (iv) a corresponding portion of an RNA sequence encoded by the endogenous senescence-induced eIF-5A gene, or (v) a combination of (I) or (ii) and (iii) or (iv);
- and
- (B) regulatory sequences operatively linked to the antisense nucleotide sequences such that the antisense nucleotides are expressed;
- and
- (2) growing said cell, whereby said antisense nucleotide sequences are transcribed and bind to said RNA sequence, whereby expression of said senescence-induced deoxyhypusine synthase gene, senescence-induced eIF-5A gene or both is inhibited.
8 more elements
A method of inhibiting seed aging, said method comprising
- (1) integrating into the genome of a plant a vector comprising
- (A) antisense nucleotide sequences substantially complementary to (i) a corresponding portion of one strand of a DNA molecule encoding an endogenous aging-induced deoxyhypusine synthase, wherein DNA encoding said endogenous aging-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 1, SEQ ID NO:
- 5 and/or SEQ ID NO:
- 9 or (ii) a corresponding portion of an RNA sequence transcribed from a DNA molecule encoding an endogenous senescence-induced deoxyhypusine synthase gene;
- and
- (B) regulatory sequences operatively linked to the antisense nucleotide sequences;
- and
- (2) growing said plant, whereby said antisense nucleotide sequences are transcribed and bind to said RNA sequence and expression of said aging-induced deoxyhypusine synthase gene is inhibited.
6 more elements
A method of inhibiting seed aging, said method comprising
- (1) integrating into the genome of a plant a vector comprising
- (A) antisense nucleotide sequences substantially complementary to (i) a corresponding portion of one strand of a DNA molecule encoding an endogenous aging-induced deoxyhypusine synthase, wherein DNA encoding said endogenous aging-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 1, SEQ ID NO:
- 5 and/or SEQ ID NO:
- 9 or (ii) a corresponding portion of a substantially complementary RNA sequence transcribed from a DNA molecule encoding an endogenous senescence-induced deoxyhypusine synthase gene, (iii) a corresponding portion of one strand of a DNA molecule encoding an endogenous aging-induced eIF-5A gene, wherein DNA encoding said endogenous aging-induced eIF-5A hybridizes with SEQ ID NO:
- 11, SEQ ID NO:
- 13 and/or SEQ ID NO:
- 15, (iv) a corresponding portion of a substantially complementary RNA sequence transcribed from a DNA molecule encoding an endogenous senescence-induced eIF-5A gene;
- or (v) a combination of (i) or (ii) and (iii) or (iv);
- and
- (B) regulatory sequences operatively linked to the antisense nucleotide sequences;
- and
- (2) growing said plant, whereby said antisense nucleotide sequences are transcribed and bind to said substantially complementary RNA sequence and expression of said aging-induced deoxyhypusine synthase gene, eIF-5A gene or both is inhibited.
10 more elements
A method of increasing seed yield from a plant, said method comprising
- (1) integrating into the genome of the plant a vector comprising
- (A) antisense nucleotide sequences substantially complementary to (i) a corresponding portion of one strand of a DNA molecule encoding an endogenous senescence-induced deoxyhypusine synthase, wherein DNA encoding said endogenous senescing-induced deoxyhypusine synthase hybridizes with SEQ ID NO:
- 1, SEQ ID NO:
- 5 and/or SEQ ID NO:
- 9 or (ii) a corresponding portion of an RNA sequence transcribed from a DNA molecule encoding an endogenous senescence-induced deoxyhypusine synthase gene;
- and
- (B) regulatory sequences operatively linked to the antisense nucleotide sequences;
- and
- (2) growing said plant, whereby said antisense nucleotide sequences are transcribed and bind to said RNA sequence and expression of said deoxyhypusine synthase gene is inhibited.
6 more elements
Dependencies are inferred from the claim text.