Genes of the 1-desoxy -d-xylulose biosynthesis path
OwnerHassan Jomaainventor
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Abstract
The invention relates to DNA sequences from Plasmodium falciparum, namely the genes lytB and yfgB which, when integrated into the genome of viruses, eukaryotes and prokaryotes, alter the isoprenoid biosynthesis. The invention also relates to gene technological methods for producing these transgenic viruses, eukaryotes and prokaryotes and to methods for identifying substances with a herbicidal, antimicrobial, antiparasitic, antiviral, fungicidal and bactericidal effect in plants and an antimicrobial, antiparasitic, antimycotic, antibacterial and antiviral effect in human beings and animals.
Claims21
DNA sequences which code for a polypeptide with the amino acid sequence shown in SEQ ID NO:
- 5 or for an analogue or derivative of the polypeptide according to SEQ ID NO:
- 5 wherein one or more amino acids have been deleted, added or replaced by other amino acids, without substantially reducing the enzymatic action of the polypeptide.
DNA sequences which code for a polypeptide with the amino acid sequence shown in SEQ ID NO:
- 14 or for an analogue or derivative of the polypeptide according to SEQ ID NO:
- 14 wherein one or more amino acids have been deleted, added or replaced by other amino acids, without substantially reducing the enzymatic action of the polypeptide.
DNA sequence with the following part sequences
- i) promoter which is active in viruses, eukaryotes and prokaryotes and ensures the formation of an RNA in the envisaged target tissue or the target cells,
- ii) DNA sequence which codes for a polypeptide with the amino acid sequence shown in SEQ ID NO:
- 5 or 14 or for an analogue or derivative of the polypeptide according to SEQ ID NO:
- 5 or 14,
- iii) 3′-nontranslated sequence which leads to the addition of poly-A radicals on to the 3′-end of the RNA in viruses, eukaryotes and prokaryotes.
Protein which participates in the 1-deoxy-D-xylulose 5-phosphate metabolic pathway and a) is coded by the DNA sequence SEQ ID NO:
- 1 or 9 or b) is coded by DNA sequences which hybridize with the DNA sequences SEQ ID NO:
- 1 or 9 or fragments of these DNA sequences in the DNA region which codes for the mature protein or c) is coded by DNA sequences which would hybridize with the sequences defined in b) without degeneration of the genetic code and code for a polypeptide with a corresponding amino acid sequence.
Method for determination of the enzymatic activity of the LytB and YfgB protein, characterized in that the change in the concentration of the substrates, co-substrates and products is determined.
Method for screening a compound, wherein the method comprises:
- a) provision of a host cell which contains a recombinant expression vector, wherein the vector has at least part of the oligonucleotide sequence according to SEQ ID NO:
- 1 or SEQ ID NO:
- 9 or variants or analogues of this, and in addition a compound which is presumed to have an antimycotic, antibiotic, antiparasitic or antiviral action in humans and animals,
- b) bringing the microorganism into contact with the compound and
- c) determination of the antimycotic, antibiotic, antiparasitic or antiviral activity of the compound.
Method for screening a compound, wherein the method comprises:
- a) provision of a host cell which contains a recombinant expression vector, wherein the vector has at least part of the oligonucleotide sequence according to SEQ ID NO:
- 1 or SEQ ID NO:
- 9 or variants or analogues of this, and in addition a compound which is presumed to have an antimycotic, antibiotic, antiparasitic or antiviral action in humans and animals,
- b) bringing the microorganism into contact with the compound and
- c) determination of the bactericidal, fungicidal or herbicidal activity of the compound.
Dependencies are inferred from the claim text.