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AssigneeBoard of Regents of the University of Nebraska
InventorsDonald P. Weeks, Xiao-Zhuo Wang, Patricia L. Herman
Estimating the term…
The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) ofthe invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation.
An isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading oxygenase.
A DNA construct comprising a DNA sequence coding for a dicamba-degrading oxygenase operatively linked to expression control sequences.
An isolated and at least partially purified dicamba-degrading oxygenase.
An isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading ferredoxin.
A DNA construct comprising a DNA sequence coding for a dicamba-degrading ferredoxin operatively linked to expression control sequences.
An isolated and at least partially purified dicamba-degrading ferredoxin.
An isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading reductase.
A DNA construct comprising a DNA sequence coding for a dicamba-degrading reductase operatively linked to expression control sequences.
An isolated and at least partially purified dicamba-degrading reductase.
A DNA construct comprising a DNA sequence coding for a dicamba-degrading oxygenase, a DNA sequence coding for a dicamba-degrading ferredoxin, and a DNA sequence coding for a dicamba-degrading reductase, each DNA sequence being operatively linked to expression control sequences.
An isolated and at least partially purified dicamba-degrading O-demethylase.
A transgenic host cell comprising DNA coding for a dicamba-degrading oxygenase operatively linked to expression control sequences.
A transgenic host cell comprising DNA coding for a dicamba-degrading ferredoxin operatively linked to expression control sequences.
A transgenic host cell comprising DNA coding for a dicamba-degrading reductase operatively linked to expression control sequences.
A transgenic host cell comprising a first DNA coding for a dicamba-degrading oxygenase and a second DNA coding for a dicamba-degrading ferredoxin, a third DNA coding for a dicamba-degrading reductase, or both the second DNA and the third DNA, each DNA being operatively linked to expression control sequences.
A transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a dicamba-degrading oxygenase operatively linked to expression control sequences.
A transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a dicamba-degrading ferredoxin operatively linked to expression control sequences.
A transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a dicamba-degrading reductase operatively linked to expression control sequences.
A transgenic plant or part of a plant comprising one or more cells comprising a first DNA coding for a dicamba-degrading oxygenase and a second DNA coding for a dicamba-degrading ferredoxin, a third DNA coding for a dicamba-degrading reductase, or both the second DNA and the third DNA, each DNA being operatively linked to expression control sequences.
A method of controlling weeds in a field containing a dicamba-tolerant transgenic plant, the method comprising applying an amount of dicamba to the field effective to control the weeds in the field.
A method of decontaminating a material containing dicamba comprising applying an amount of a dicamba-degrading transgenic microorganism to the material, the amount being effective to degrade at least some of the dicamba in the material.
A method of decontaminating a material containing dicamba comprising applying an amount of a dicamba-degrading O-demethylase or of a combination of a dicamba-degrading oxygenase, a dicamba-degrading ferredoxin and a dicamba-degrading reductase to the material, the amount being effective to degrade at least some of the dicamba in the material.
A method of selecting transformed plant cells comprising:
A method of selecting transformed plants comprising:
A method of selecting, or screening for, transformed host cells, intact organisms, and parts of organisms, the method comprising:
Dependencies are inferred from the claim text.