Methods and compositions for producing donor cells, as well as tissues and organs comprising the same
OwnerHal Sternberginventor
Life of this patent
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Abstract
Methods and compositions are provided for producing at least one donor cell that has a genome identical to that of a host. In the subject methods, an embryo is first produced by combining a nucleus obtained for a cell of said host with a recipient cell, e.g. an enucleated oocyte. The resultant embryo is then matured under conditions sufficient to produce a non-sentient organism that includes the donor cell. Finally, the donor cell is harvested from the non-sentient organism for subsequent use. Also provided are kits for use in practicing the subject invention. The subject methods find use in a variety of different applications, including tissue and organ transplant applications.
Claims22
A method of producing at least one donor cell having a genome identical to that of a host, said method comprising:
- producing an embryo by transferring a nucleus obtained from a cell of said host into a recipient cell;
- maturing said embryo under conditions sufficient to produce a non-sentient organism comprising said at least one donor cell;
- and
- harvesting said at least one donor cell from said non-sentient organism;
- whereby said at least one donor cell having a genome identical to that of said host is produced.
A method of producing mammalian tissue, said method comprising:
- producing an embryo by transferring a nucleus obtained from a cell of host into an enucleated oocyte;
- maturing said embryo under conditions sufficient to produce a non-sentient organism comprising said tissue, wherein said non-sentient organism lacks a functional forebrain;
- and
- harvesting said tissue from said non-sentient organism;
- whereby said tissue is produced.
A method of transplanting donor mammalian tissue to a mammalian recipient, said method comprising:
- (a) producing said donor mammalian tissue by the method comprising:
- (i) producing an embryo by transferring a nucleus obtained from a cell of a host into an enucleated oocyte;
- (ii) maturing said embryo under conditions sufficient to produce a non-sentient organism comprising said donor mammalian tissue, wherein said non-sentient organism lacks a functional forebrain;
- and
- (iii) harvesting said donor mammalian tissue from said non-sentient organism, whereby said tissue is produced;
- and
- (b) transplanting said harvested donor mammalian tissue to said mammalian recipient;
- whereby donor mammalian tissue is transplanted to said mammalian recipient.
Dependencies are inferred from the claim text.