Transgenic mice comprising a genomic human tau transgene
OwnerKaren Duffinventor
Life of this patent
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Abstract
The invention provides transgenic mice comprising tau transgenes, and methods of preparing and using the transgenic mice.
Claims53
A transgenic mouse, the genome of the cells of which comprise a DNA molecule which comprises a human genomic DNA sequence encoding tau, wherein the DNA sequence is stably integrated into the genome of the cells of the mouse, and wherein the DNA sequence is expressed in the transgenic mouse so as to result in the transgenic mouse expressing at least one isoform of human tau.
An isolated and purified DNA segment which comprises a human genomic DNA sequence encoding tau.
A method for expression of human tau in a mouse, comprising preparing a transgenic mouse, the genome of the cells of which comprise a human genomic DNA sequence which encodes tau so as to result in the expression of at least one isoform of human tau in the transgenic mouse.
A method of using a transgenic mouse to screen for an agent that reduces or inhibits a neurodegenerative disorder, comprising:
- (a) administering the agent to the transgenic mouse, wherein the genome of the cells of the transgenic mouse comprise a human genomic DNA sequence encoding tau, wherein the DNA sequence is integrated into the genome of the mouse, and wherein the DNA segment is expressed in the transgenic mouse so as to result in the expression of at least one isoform of human tau;
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- (b) determining whether the agent reduces or inhibits a neurodegenerative disorder in the transgenic mouse relative to a transgenic mouse of step (a) which has not been administered the agent.
A transgenic mouse, the genome of the cells of which comprise a recombinant DNA molecule which comprises a humanized murine DNA sequence encoding tau, wherein the DNA sequence is stably integrated into the genome of the cells of the mouse, and wherein the DNA sequence is expressed in the transgenic mouse so as to result in the transgenic mouse expressing the humanized murine tau.
A transgenic mouse, the genome of the cells of which stably comprise a DNA molecule which comprises a human genomic DNA sequence comprising a human tau promoter and which DNA sequence encodes human tau, wherein the DNA sequence comprises one SrfI restriction site in the tau coding region, and wherein the DNA sequence is expressed in the transgenic mouse so as to result in the transgenic mouse expressing six isoforms of human tau.
An isolated and purified DNA segment which comprises a human genomic DNA sequence comprising a human tau promoter and which DNA sequence encodes human tau.
A transgenic mouse, the genome of the cells of which stably comprise a recombinant DNA molecule which comprises a humanized murine DNA sequence encoding tau, wherein the DNA sequence is expressed in the transgenic mouse so as to result in the transgenic mouse expressing the humanized murine tau.
Dependencies are inferred from the claim text.