Method of generating a transgenic livestock animal
AssigneeThe Regents of the University of California
InventorsJames D. Murray, Elizabeth A. Maga, Gary B. Anderson, Stefanie M. Oppenheim
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Abstract
The present invention provides methods of producing transgenic livestock animals. The methods generally involve first introducing a nucleoprotein made up of nucleic acid and a recombinase into a totipotent or pluripotent cell to produce a recombinant totipotent or pluripotent cell and then growing the recombinant totipotent or pluripotent cell to produce the transgenic livestock animal. The invention further provides kits for use in generating transgenic non-human animals of the invention.
Claims20
A method of producing a transgenic livestock animal, said method comprising:
- (a) introducing a nucleoprotein made up of nucleic acid and a recombinase into a totipotent or pluripotent cell to produce a recombinant totipotent or pluripotent cell;
- and
- (b) growing said recombinant totipotent or pluripotent cell to produce said transgenic, non-human animal.
A nucleoprotein comprising a recombinase and a nucleic acid, wherein said nucleic acid includes a homology clamp for a livestock genome.
Dependencies are inferred from the claim text.