Transgenic animal having a disrupted PDE7A gene and uses thereof
AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorTamar Michaeli
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Abstract
The present invention provides a transgenic non-human animal whose genome comprises a disruption in its endogenous PDE7A gene, wherein the transgenic animal exhibits decreased expression of functional PDE7A protein relative to wild-type. The present invention further provides a method for creating a transgenic non-human animal exhibiting decreased expression of functional PDE7A protein relative to wild-type. Finally, the present invention provides a method for screening a PDE7A inhibitor for at least one side-effect.
Claims20
A transgenic non-human animal whose genome comprises a disruption in its endogenous PDE7A gene, wherein the transgenic animal exhibits decreased expression of functional PDE7A protein relative to wild-type.
A method for creating a transgenic non-human animal exhibiting decreased expression of functional PDE7A protein relative to wild-type, comprising the steps of:
- (a) generating a PDE7A targeting vector;
- (b) introducing the PDE7A targeting vector into a recipient cell of a non-human animal, to produce a treated recipient cell;
- (c) introducing the treated recipient cell into a blastocyst of a non-human animal, to produce a treated blastocyst;
- (d) introducing the treated blastocyst into a pseudopregnant non-human animal;
- (e) allowing the transplanted blastocyst to develop to term;
- (f) identifying a transgenic non-human animal whose genome comprises a disruption in its endogenous PDE7A gene;
- and
- (g) breeding the transgenic non-human animal to obtain a transgenic non-human animal exhibiting decreased expression of functional PDE7A protein relative to wild-type.
Dependencies are inferred from the claim text.