Antibody composition-producing cell
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsYutaka Kanda, Mitsuo Satoh, Kazuyasu Nakamura, Kazuhisa Uchida, Toyohide Shinkawa, Naoko Yamane, Emi Hosaka, Kazuya Yamano, Motoo Yamasaki, Nobuo Hanai
Life of this patent
Estimating the term…
Abstract
The present invention relates to a cell for the production of an antibody molecule such as an antibody useful for various diseases having high antibody-dependent cell-mediated cytotoxic activity, a fragment of the antibody and a fusion protein having the Fc region of the antibody or the like, a method for producing an antibody composition using the cell, the antibody composition and use thereof.
Claims61
A Chinese hamster ovary tissue-derived CHO cell into which a gene encoding an antibody molecule is introduced, which produces an antibody composition comprising an antibody molecule having complex N-glycoside-linked sugar chains bound to the Fc region, wherein among the total complex N-glycoside-linked sugar chains bound to the Fc region in the composition, the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain is 20% or more.
An antibody composition which comprises an antibody molecule having complex N-glycoside-linked sugar chains bound to the Fc region which is produced by a CHO cell, wherein among the total complex N-glycoside-linked sugar chains bound to the Fc region in the composition, the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain is 20% or more.
A cell in which the activity of an enzyme relating to the synthesis of an intracellular sugar nucleotide, GDP-fucose and/or the activity of an enzyme relating to the modification of a sugar chain wherein 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in the complex N-glycoside-linked sugar chain is decreased or deleted by a genetic engineering technique.
A transgenic non-human animal or plant or the progenies thereof, comprising a genome which is modified such that the activity of an enzyme relating to the synthesis of an intracellular sugar nucleotide, GDP-fucose and/or the activity of an enzyme relating to the modification of a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in the N-glycoside-linked sugar chain is decreased.
A protein selected from the group consisting of the following (a), (b), (c), (d), (e), (f), (g), (h), (i) and (j):
- (a) a protein comprising the amino acid sequence represented by SEQ ID NO:71;
- (b) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO:71 and has GMD activity;
- (c) a protein comprising the amino acid sequence represented by SEQ ID NO:72;
- (d) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO:72 and has Fx activity;
- (e) a protein comprising the amino acid sequence represented by SEQ ID NO:73;
- (f) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO:73 and has GFPP activity;
- (g) a protein comprising the amino acid sequence represented by SEQ ID NO:23;
- (h) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO:23 and has α-1,6-fucosyltransferase activity;
- (i) a protein comprising the amino acid sequence represented by SEQ ID NO:24;
- (j) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO:
- 24 and the α-1,6-fucosyltransferase activity.
A DNA selected from the group consisting of the following (a), (b), (c), (d) and (e):
- (a) a DNA comprising the nucleotide sequence represented by SEQ ID NO:1;
- (b) a DNA comprising the nucleotide sequence represented by SEQ ID NO:2;
- (c) a DNA comprising the nucleotide sequence represented by SEQ ID NO 65;
- (d) a DNA comprising the nucleotide sequence represented by SEQ ID NO:48;
- (e) a DNA comprising the nucleotide sequence represented by SEQ ID NO:51.
A genome DNA selected from the group consisting of the following (a), (b) and (c):
- (a) a genome DNA comprising the nucleotide sequence represented by SEQ ID NO:3;
- (b) a genome DNA comprising the nucleotide sequence represented by SEQ ID NO:67;
- (c) a genome DNA comprising the nucleotide sequence represented by SEQ ID NO:70.
Dependencies are inferred from the claim text.