Phytochelatin synthase
AssigneeE. I. Du Pont de Nemours and Company
InventorsKarlene H. Butler, Omolayo O. Famodu, Leslie T. Harvell, Emil M. Orozco JR., Sonriza Rasco-Gaunt, Catherine J. Thorpe
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Abstract
The present invention relates to an isolated nucleic acid fragment encoding a phytochelatin synthase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the phytochelatin synthase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the phytochelatin synthase in a transformed host cell.
Claims21
An isolated polynucleotide comprising:
- (a) a first nucleotide sequence encoding a first polypeptide having phytochelatin synthase activity, wherein the amino acid sequence of the first polypeptide and the amino acid sequence of SEQ ID NO:12 or SEQ ID NO:18 have at least 70% sequence identity based on the ClustaIV alignment method,
- (b) a second nucleotide sequence encoding a second polypeptide having phytochelatin synthase activity, wherein the amino acid sequence of the second polypeptide and the amino acid sequence of SEQ ID NO:14 or SEQ ID NO:16 have at least 80% sequence identity based on the ClustaIV alignment method, or
- (c) the complement of the nucleotide sequence of (a) or (b).
An isolated polypeptide having phytochelatin synthase activity, wherein the polypeptide comprises:
- (a) a first amino acid sequence, wherein the first amino acid sequence and the amino acid sequence of SEQ ID NO:12 or SEQ ID NO:18 have at least 70% sequence identity based on the ClustaIV alignment method, or
- (b) a second amino acid sequence, wherein the second amino acid sequence and the amino acid sequence of SEQ ID NO:14 or SEQ ID NO:16 have at least 80% sequence identity based on the ClustaIV alignment method.
Dependencies are inferred from the claim text.