Antithrombin nucleotides and proteins from horn fly
AssigneeAuburn University
InventorsEddie Wayne Cupp, Mary Smith Cupp
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Abstract
Compositions and methods for preventing hematophagous infestation of cattle are provided, directed at isolated proteins with antithrombin activity and nucleotide sequences encoding the proteins. The protein named thrombostasin is isolated from the salivary glands of Haematobia irritans. The compositions are useful as veterinary vaccines in prevention of blood-feeding in cattle by the infesting horn fly. The proteins of the invention are also useful in treatment of thrombosis.
Claims12
A substantially purified protein having antithrombin activity, wherein said protein is isolated from the salivary glands of a species of the suborder Cyclorrhapha.
A substantially purified protein having the amino acid sequence selected from the group consisting of:
- a) an amino acid sequence of an antithrombin protein from a species of the suborder Cyclorrhapha;
- b) an amino acid sequence that is at least 80% identical to the amino acid sequence set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- c) an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- and
- d) an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- and
- e) an amino acid sequence set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 5, or SEQ ID NO:
- 7.
A fragment of the amino acid sequence according to SEQ ID NO:2, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- wherein said fragment comprises at least 15 contiguous amino acids of said sequence.
Dependencies are inferred from the claim text.