Methods and reagents for preserving RNA in cell and tissue samples
OwnerEric S. Laderinventor
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Abstract
This specification relates to the field of molecular biology and provides novel methods and reagents for preserving and protecting the ribonucleic acid (RNA) content of samples from degradation prior to RNA isolation. This preservation may be accomplished without ultra-low temperature storage or disruption of the tissue.
Claims63
A method of preserving RNA comprising:
- (i) obtaining an RNA-containing sample;
- and
- (ii) treating the sample with an RNA preservation medium that infiltrates the sample and protects the RNA from nucleases.
A kit for preserving RNA within a sample and isolating the RNA from the sample comprising:
- (i) an RNA preservation medium that infiltrates the sample and protects the RNA from nucleases;
- and
- (ii) a reagent for performing an RNA extraction from the sample.
An RNA preservation medium that comprises a sulfate salt.
A method of preserving RNA comprising:
- (i) obtaining an RNA-containing sample;
- (ii) providing a salt;
- and
- (iii) admixing the sample and the salt in a liquid to form an RNA preservation composition that infiltrates the sample and protects the RNA from nucleases.
The method of claim S1, wherein said buffer has a pH between 4 and 8.
A composition of matter comprising an RNA-containing sample, a liquid, and a salt in a concentration sufficient to protect the RNA from nucleases.
Dependencies are inferred from the claim text.