Plant growth factor
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsYouji Sakagami, Yoshikatsu Matsubayashi
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Abstract
The present invention relates to a peptide of the formula: 1 wherein R1 and R2 are the same or different and each represents SO3H or H; X represents an α-amino acid or a single bond; Z1 and Z2 are the same or different and each represents an α-amino acid; and Y represents OH or NH2. This peptide has plant growth factor properties.
Claims19
A peptide of the formula:
- 5
- wherein R1 and R2 are the same or different and each represents SO3H or H;
- X represents an α-amino acid or a single bond;
- Z1 and Z2 are the same or different and each represents an α-amino acid;
- and Y represents OH or NH2.
A peptide of the formula:
- 6
- wherein X represents glutamine or a single bond;
- and Y represents OH or NH2.
A plant growth factor obtained by collecting cells from liliaceous plants, incubating the collected cells in a plant cell cultivation medium, and separating said plant growth factor from the cells through centrifugation.
A plant growth factor, wherein the plant growth factor has the following physico-chemical properties:
- a) it is easily soluble in water, but is hardly soluble in ethanol and acetone;
- b) it is acidic;
- c) it keeps 70% of its activity, after being heated at 100° C. for 10 minutes and it is deactivated, after being autoclaved at 121° C. for 20 minutes;
- d) it is a polar substance, and is not retained in reversed-phase columns with Cosmosil 75C18-OPN and Diaion HP-20;
- e) it is stable at pH of 3 to 9, but at pH 11, its activity is reduced to 60%;
- f) it is deactivated by Pronase E, but its activity is still kept even when treated with Glycosidases “Mixed”;
- and
- g) it is strongly adsorbed to DEAE Sephadex A-25 ion-exchange resin (and eluted with 1000 mM KCl), but it is not adsorbed at all to CM Sephadex C-25.
Dependencies are inferred from the claim text.