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AssigneeNihon Shokuhin Kako Co., Ltd.
InventorsMasayasu Takada, Takahiro Ide, Yoshinori Nakagawa, Takeshi Yamamoto, Mikio Yamamoto
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The invention provides a gene (isolated nucleic acid molecule) encoding the cyclodextrin glucanotransferase which produces a considerable amount of γ-cyclodextrin (γ-CD) from the substrates selected from among starch and starch decomposition products such as dextrin, amylopectin and amylose; recombinant plasmids comprising this gene; transformants transformed with the recombinant plasmid; methods of manufacturing the cyclodextrin glucanotransferase by employing these transformants to act upon the substrates selected from among starch and decomposition products thereof and causing the production of γ-CD as a main product; and methods of manufacturing γ-CD and CD-comprising compositions having a desired CD balance (α-, β- and γ-CD balance) employing this recombinant cyclodextrin glucanotransferase.
An isolated nucleic acid molecule encoding a protein having the amino acid sequence of amino acid residues 1 to 702 denoted by SEQ ID No. 2 in the attached Sequence Listing, or an amino acid sequence consisting of said amino acid sequence in which one or multiple amino acids have been substituted, inserted, added or deleted;
An isolated nucleic acid molecule encoding a protein having the amino acid sequence of amino acid residues 29 to 702 denoted by SEQ ID No. 2 in the attached Sequence Listing, or an amino acid sequence consisting of said amino acid sequence in which one or multiple amino acids have been substituted, inserted, added or deleted;
An isolated nucleic acid molecule having the nucleotide sequence of nucleotides 321 to 2,426 or the nucleotide sequence of nucleotides 405 to 2,426 of SEQ ID No. 1 of the attached Sequence Listing.
A protein having the amino acid sequence of amino acid residues 1 to 702 denoted by SEQ ID No. 2 in the Sequence Listing, or an amino acid sequence consisting of said amino acid sequence in which one or multiple amino acids have been substituted, inserted, added or deleted;
A protein having the amino acid sequence of amino acid residues 29 to 702 denoted by SEQ ID No. 2 in the Sequence Listing, or an amino acid sequence consisting of said amino acid sequence in which one or multiple amino acids have been substituted, inserted, added or deleted;
Dependencies are inferred from the claim text.