US 2003/0114373 A1 · Polynucleotide encoding a novel cysteine protease of the calpain superfamily, CAN-12, and variants thereof

Abstract

The present invention provides novel polynucleotides encoding CAN-12 polypeptides, fragments and homologues thereof. The present invention also provides polynucleotides encoding variants of CAN-12 polypeptides, CAN-12v1 and CAN-12v2. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel CAN-12, CAN-12v1, and CAN-12v2 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides, particularly neuro- and musculo-degenerative conditions. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95.0% identical to a sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:55 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:56 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:55;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:56 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:55;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:56 or a polypeptide epitope encoded by the cDNA sequence included in ATCC deposit no:
PTA-3434, which is hybridizable to SEQ ID NO:55;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:56 or the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:55, having calpain activity;
(f) a polynucleotide which is a variant of SEQ ID NO:55;
(g) a polynucleotide which is an allelic variant of SEQ ID NO:55;
(h) an isolated polynucleotide comprising nucleotides 12 to 2099 of SEQ ID NO:55, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 697 of SEQ ID NO:56 minus the start codon;
(i) an isolated polynucleotide comprising nucleotides 9 to 2099 of SEQ ID NO:55, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 697 of SEQ ID NO:56 including the start codon;
(j) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:55;
(k) a polynucleotide fragment of SEQ ID NO:53 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:53;
(l) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:54 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:53;
(m) a polynucleotide encoding a polypeptide domain of SEQ ID NO:54 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:53;
(n) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:54 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:53;
(o) a polynucleotide encoding a polypeptide of SEQ ID NO:54 or the cDNA sequence included in ATCC Deposit No:
PTA-3434, which is hybridizable to SEQ ID NO:53, having calpain activity;
(p) an isolated polynucleotide comprising nucleotides 12 to 2090of SEQ ID NO:53, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 694 of SEQ ID NO:54 minus the start codon;
(q) isolated polynucleotide comprising nucleotides 9 to 2090 of SEQ ID NO:53, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 694 of SEQ ID NO:54 including the start codon;
(r) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:53;
(s) an isolated polynucleotide comprising nucleotides 117 to 1995 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 581 of SEQ ID NO:2 minus the start codon;
(t) an isolated polynucleotide comprising nucleotides 114 to 1995 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 581 of SEQ ID NO:2 including the start codon;
(u) an isolated polynucleotide comprising nucleotides 117 to 1397 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 428 of SEQ ID NO:2 minus the start codon;
(v) an isolated polynucleotide comprising nucleotides 114 to 1397 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 428 of SEQ ID NO:2 including the start codon;
(w) an isolated polynucleotide comprising nucleotides 1537 to 1995 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 429 to 581 of SEQ ID NO:2;
(x) an isolated polynucleotide comprising nucleotides 132 to 1397 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 7 to 428 of SEQ ID NO:2;
(y) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:1;
and
(z) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(y), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

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Polynucleotide encoding a novel cysteine protease of the calpain superfamily, CAN-12, and variants thereof

Life of this patent

Polynucleotide encoding a novel cysteine protease of the calpain superfamily, CAN-12, and variants thereof

Life of this patent

Abstract

Claims23