Integrated protein affinity capture centrifugation device
AssigneeSerenex, Inc.
InventorsTimothy A. J. Haystead, George J. Young
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Abstract
A method and apparatus are described for screening a proteome comprising an array of affinity elements, whereby a proteome is directed through the affinity array and those components that specifically bind to the affinity array are eluted in a suitable eluent by means of centrifugation. Suitable eluents can include a proteome component, a chemical library component or an affinity element. This method and apparatus can be used for several purposes, including screening for bio-active compounds, determining physiological targets of known drugs, determining specificity of compound interactions with physiological targets, and for quantitative protein analyses.
Claims69
A method for screening a proteome, comprising the steps of:
- a) directing compounds of a proteome through an array of affinity elements, whereby at least one compound of the proteome binds specifically to at least one of said affinity elements;
- b) washing the array, whereby non-specifically bound compounds of the proteome are eluted from the array;
- and
- c) directing at least one eluent through the array by centrifugal force, wherein said eluent releases said bound compound, whereby said compound is eluted from the affinity element as a component of an eluate, thereby screening the proteome.
An apparatus for screening a proteome, comprising:
- a) an array of affinity elements, said affinity elements including at least one ligand;
- and
- b) means for applying centrifugal force to said array, whereby an eluent can be directed through the array by centrifugal force and can release a compound of the proteome that is bound to the ligand, thereby eluting the compound from the affinity element as a component of an eluate and screening the proteome.
Dependencies are inferred from the claim text.