Nucleic acid sequences capable of improving homologous recombination in plants and plant plastids
OwnerLarry A. Gilbertson, Jeffrey M. Staubinventors
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Abstract
A method for improved plastid transformation efficiency via homologous recombination and nucleic acid sequences useful therefore is provided. Nucleic acid sequences comprising a 5 base pair recombination sequence motif or multiple direct repeats thereof that increase the frequency of integration of a selected transgene through plastid transformation by homologous recombination are provided.
Claims11
A nucleic acid recombination sequence motif selected from the group consisting of 5′-TATTA-3′ and 3′-TAATA-5′ and single nucleotide variations of said motifs.
A nucleic acid sequence comprising a plurality of nucleic acid recombination sequence motifs selected from the group consisting of (TATTA)n and (TAATA)n, where n is between 2 and 40.
A nucleic acid recombination sequence motif comprising an ArTr repeated sequence where r is between about 5 and about 50 and said ArTr repeated sequence is between about 20 and about 100 base pairs in length.
A nucleic acid sequence for plastid transformation comprising a plastid functional promoter operably linked to a transgene which is operably linked to a transcript termination region forming an expression cassette, said expression cassette flanked by a plastid region of homology comprising a nucleic acid recombination sequence motifs selected from the group consisting of (TATTA)n and (TAATA)n, where n is between 2 and 40.
A transplastomic plant comprising a recombination sequence motif integrated into its plastid genome for use as a transformation integration site, said recombination sequence motif selected from the group consisting of (TATTA)n and (TAATA)n, where n is between 2 and 40.
Dependencies are inferred from the claim text.