Methods for delivering nucleic acid molecules into cells and assessment thereof
AssigneeChromos Molecular Systems, Inc.
InventorsGary de Jong, Sandra Louise Vanderbyl, Jan Drayer, Paul Peter Tak
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Abstract
Methods for delivering nucleic acid molecules into cells and methods for measuring nucleic acid delivery into cells and the expression of the nucleic acids are provided. The methods are designed for introduction of large nucleic acid molecules, including artificial chromosomes, into cells, and are practiced in vitro and in vivo.
Claims56
A synoviocyte comprising heterologous nucleic acid that comprises at least about 0.5 megabase pairs (Mbase).
A synoviocyte comprising an artificial chromosome.
A method for introducing heterologous nucleic acid into a synoviocyte, comprising introducing an artificial chromosome into the synoviocyte.
A method for introducing a large nucleic acid molecule into a synoviocyte, comprising:
- (a) exposing the nucleic acid molecule to a delivery agent;
- and
- (b) contacting the synoviocyte with the nucleic acid molecule.
A method for delivering a nucleic acid molecule into a synoviocyte comprising:
- (a) contacting the synoviocyte in the absence of the nucleic acid molecule with a delivery agent, and applying ultrasound energy or electrical energy to the synoviocyte, wherein the contacting and applying are performed sequentially or simultaneously;
- and then
- (b) contacting the synoviocyte with the nucleic acid molecule, whereby the nucleic acid molecule is delivered into the synoviocyte.
A method for delivering a nucleic acid molecule into a synoviocyte comprising:
- (a) contacting the synoviocyte with the nucleic acid molecule and a delivery agent, and applying ultrasound energy or electrical energy to the synoviocyte, wherein the contacting and applying are performed sequentially or simultaneously and whereby the nucleic acid molecule is delivered into the synoviocyte.
A method for modulating a rheumatic disease process in a subject, comprising, introducing a large nucleic acid into the subject;
- wherein the large nucleic acid comprises nucleic acid that is or that encodes an agent that modulates a rheumatic disease process.
A method for identifying candidate agents for the treatment of a connective tissue disease, comprising:
- introducing a large nucleic acid molecule comprising nucleic acid that is or encodes a candidate agent into an animal model of the disease;
- and
- determining if the agent ameliorates one or more conditions of the disease in the animal.
A composition, comprising cells selected for therapeutic treatment of a joint, wherein the cells comprise a large heterologous nucleic acid.
A composition, comprising cells selected for therapeutic treatment of rheumatoid arthritis, wherein the cells comprise a large heterologous nucleic acid.
Dependencies are inferred from the claim text.