Method for producing somatic embryos of scot pine (p sylvestris)
OwnerCarlos Ramirez Serranoinventor
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Abstract
A method for producing mature somatic embryos, such method was improved with cryopreserved genotypes and it was applied successfully in a wide range of genotypes and families This method comprises the establishment and continuing proliferation of at least 40% of induced genotypes, treatment for reducing proliferation rates, treatment for initiating the development of immature somatic embryo, somatic embryo maturation of at least 80% of the treated genotypes belonging at least 90% of the tested families. Such result were achieved by simultaneously application of different ammonium to nitrate molar rations depending on the step which are included in the present method.
Claims39
A method for producing mature somatic embryos from a wide range of cryopreserved or no cryopreserved genotypes belonging to different families, by using solid medium in all the steps of the process This method comprises the establishment of embryogenic tissue, continuing proliferation, the treatment to reduce the proliferation, the treatment to initiate the immature somatic embryo development, and the somatic embryo maturation
A gymnosperm mature somatic embryo characterized by giving the ancestral immature somatic embryo a establishment proliferation treatment through subculturing at least in two media, a high quality proliferation at least in two media, a reduction of proliferation rate previous to maturation treatment according with the genotype used, having a treatment to start the development of somatic embryo and having maturation treatment by using a medium supplemented with a low ammonium to nitrate molar ratio, a carbon source, a maturation promoter, and a desiccant compound.
Dependencies are inferred from the claim text.