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US 2003/0113910 A1Published application
Pluripotent stem cells derived without the use of embryos or fetal tissue
OwnerMike Levanduskiinventor
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OwnerMike Levanduskiinventor
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This invention provides a method for deriving precursors to pluripotent non-embryonic stem (P-PNES) and pluripotent non-embryonic stem (PNES) cell lines. The present invention involves nuclear transfer of genetic material from a somatic cell into an enucleated, zona pellucida free human ooplastoid having a reduced amount of total cytoplasm. The present invention provides a new source for obtaining human and other animal pluripotent stem cells. The source utilizes as starting materials an oocyte and a somatic cell as the starting materials but does not require the use, creation and/or destruction of embryos or fetal tissue and does not in any way involve creating a cloned being. The oocyte never becomes fertilized and never develops into an embryo. Rather, portions of the oocyte cytoplasm are extracted and combined with the nuclear material of individual mature somatic cells in a manner that precludes embryo formation. Murine, bovine, and human examples of the procedure are demonstrated. Subsequently, the newly constructed P-PNES cells are cultured in vitro and give rise to PNES cells and cell colonies. Methods are described for culturing the P-PNES cells to yield purified PNES cells which have the ability to differentiate into cells derived from mesoderm, endoderm, and ectoderm germ layers. Methods are described for maintaining and proliferating PNES cells in culture in an undifferentiated state. Methods and results are described for analysis and validation of pluripotency of PNES cells including cell morphology, cell surface markers, pluripotent tumor development in SCID mouse, karyotyping, immortality in in vitro culture.
A purified preparation of pluripotent non-embryonic stem cells, which
A purified preparation of pluripotent non-embryonic stem cells, which
A stem cell which does not originate from a fertilized egg, but which originates from the combination of a somatic cell nucleus and an enucleated ooplastoid.
A stem cell which is produced by the method of (i) contacting a desired somatic cell or somatic cell nucleus with an ooplastoid, wherein said ooplastoid is derived from an enucleated oocyte;
A nascent cell produced from the combination of a somatic cell nucleus and an enucleated zona pellucida free ooplastoid.
A method of producing pluripotent, non-embryonic stem cells comprising the following steps:
A method of producing pluripotent non-embryonic stem cells comprising the following steps:
A method of producing an ooplastoid comprising the following steps:
Dependencies are inferred from the claim text.