Culturing chamber on microscope stage
AssigneeNational Cheng Kung University
InventorsChung-Liang Ho, Tun-Yi Mou
Life of this patent
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Abstract
A culturing chamber is a closed system to culture cells on a microscope stage for a long time and consists of base (10), a compartment layer (20), and a lid (30) sealingly and detachably combined together. The lid (30) is able to be selectively opened or closed to make the operation such as cell injection convenient. An electronic signal device containing a mult-operational system is applied to the culture chamber to maintain the living circumstance steady for the cells, so that the cultured cells can live long on the microscope stage and be observed and cultured at the same time.
Claims11
A culturing chamber comprising:
- a base (10) with a top face and a bottom face having:
- a hole defined in the base (10);
- a circuit board (14) having a central hole and partially covering the hole of the base (10) by attaching to the bottom face of the base (10);
- a conductive bottom plate (12) mounted on the top face of the base (10) to cover the central hole in the circuit board (14);
- and
- a conductive sheet (142) extends between the circuit board (14) and the bottom plate (12);
- a compartment layer (20) hermetically mounted on the base (10) and having
- an inner wall (21) erected on the base (10) around the bottom plate (12) to construct an inner tank (A);
- a sealing wall (22) erected around the inner wall (21) on the base (10) and keeps a distance to the inner wall (21) to construct a water sink (B), wherein the water sink (B) communicates with the inner tank (A) and adapted to store water to provide a thermal preservative efficiency to the culturing chamber;
- a heating wall (24) erected around the sealing wall (22) and having a heating resistor (242) and a thermal sensor (244) both embedded in the heating wall (24) and both communicated with the circuit board (14);
- and
- a thermal preservative wall (26) erecting adjacent to the heating wall (24) to keep the culturing chamber constantly warm;
- a lid (30) mounted on the compartment layer (20) and having
- an opening (32) defined in the lid (30) to align with the inner tank (A);
- and
- a window (34) mounted on the lid (30) to cover the opening (32) and composed of at least one conductive glass, the lid (30) having at least two apertures (38) and at least two gas apertures (39) defined in the lid (30) to communicate with the inner tank (A), wherein each aperture (38) and gas aperture (39) has a guiding tube (382, 392) inserting into the culturing liquid;
- and
- an electronic signal device (40) connected with base (10), the compartment layer (20) and the lid (30) of the culturing chamber to automatically control the temperature, humidity, gas change and liquid change in the culturing chamber and to keep the cell growth in a desired circumstance.
Dependencies are inferred from the claim text.