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US 2003/0113900 A1Published application
Methods of preserving prokaryotic cells and compositions obtained thereby
OwnerAlan G. Tunnacliffe, David T. Welsh +3inventors
InventorsAlan G. Tunnacliffe, David T. Welsh, Bruce J. Roser, Kamaljit S. Dhaliwal, Camilo Colaco
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Abstract
This invention provides methods of drying and stabilizing prokaryotic cells, and the compositions obtained thereby. The cells are first cultured or incubated under conditions sufficient to induce intracellular trehalose, suspended in a stabilizing solution and dried to form a solid glass. The resulting product is storage-stable at room temperature, showing little viability loss on storage.
Claims24
A method of preserving prokaryotic cells comprising the steps of:
- a) increasing intracellular trehalose concentration in the prokaryotic cells to an amount effective to increase storage stability;
- b) mixing the prokaryotic cells obtained in step a) with a drying solution comprising a stabilizing agent;
- and
- c) drying the product of step b) under conditions sufficient to produce a glass form of the stabilizing agent having less than about 5% residual moisture.
1 more element
Dependencies are inferred from the claim text.