Proteases from gram-positive organisms
AssigneeGenencor International, Inc.
InventorDavid A. Estell
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Abstract
The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
Claims22
A gram-positive microorganism having a mutation or deletion of part or all of the nucleic acid encoding MP, said mutation or deletion resulting in the inactivation of the MP proteolytic activity.
A cleaning composition comprising a gram positive microorganism MP, wherein said MP is encoding by nucleic acid located immediately downstream of the transcriptional terminator of the major alkaline protease of the gram positive microorganism.
A cleaning composition comprising a gram-positive microorganism MP, wherein said MP has the amino acid sequence as shown in FIGS. 1A-1E.
An expression vector comprising nucleic acid encoding a gram-positive MP.
A method for detecting a gram-positive microorganism MP, comprising the steps of
- (a) hybridizing gram-positive microorganism nucleic acid under low stringency conditions to a probe, wherein the probe comprises part or all of the nucleic acid sequence shown in FIGS. 1A-1E;
- and
- (b) isolating gram-positive nucleic acid which hybridizes to said probe.
Dependencies are inferred from the claim text.