FARS1, a human secreted protein and uses thereof
AssigneeMillennium Pharmaceuticals, Inc.
InventorsRuth E. Gimeno, Mehran M. Khodadoust
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Abstract
The invention provides isolated nucleic acids molecules, designated FARS1 nucleic acid molecules, which encode novel secreted proteins. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing FARS1 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a FARS1 gene has been introduced or disrupted. The invention still further provides isolated FARS1 proteins, fusion proteins, antigenic peptides and anti-FARS1 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.
Claims22
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising a nucleotide sequence which is at least 80% identical to the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, or SEQ ID NO:8;
- b) a nucleic acid molecule comprising a fragment of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, or SEQ ID NO:8;
- c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10, or a biologically active fragment thereof;
- d) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10;
- and
- e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, or a complement thereof, under stringent conditions.
The host cell of claim 5 which is a mammalian host cell.
The host cell of claim 6 which is a non-human mammalian host cell.
An isolated polypeptide selected from the goup consisting of:
- a) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 80% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, or SEQ ID NO:8;
- b) a polypeptide comprising an amino acid sequence which is at least 80% identical to the amino acid sequence shown in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10;
- c) a naturally occurring variant of a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10;
- d) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which hybridizes under stringent hybridization conditions to a complement of a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, or SEQ ID NO:8;
- and
- e) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10.
A method for producing a polypeptide, wherein the method comprises introducing into a host cell a nucleic acid molecule encoding a polypeptide sequence selected from the group consisting of:
- a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10;
- b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:10;
- and
- c) a naturally occurring variant of the FARS1 polypeptide which is encoded by a nucleic acid molecule which hybridizes under stringent conditions to a complement of a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, or SEQ ID NO:8.
Dependencies are inferred from the claim text.