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US 2003/0113884 A1Published application
Methods for the biosynthesis of polyesters
AssigneeMetabolix, Inc.
InventorsSILKE HEIN, BRIGITTE SOHLING, GERHARD GOTTSCHALK, ALEXANDER STEINBUCHEL
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Abstract
Coexpression of a polyhydroxyalkanoic acid synthase and either a fatty acid: acyl-CoA transferase or an acyl-CoA synthetase in cells enables the biosynthesis of polyester materials. Plasmids, bacteria, materials, and methods for the preparation of polyesters are disclosed.
Claims61
An isolated nucleic acid segment encoding:
- a) a polyhydroxyalkanoic acid synthase protein;
- and
- b) a fatty acid:acyl-CoA transferase protein.
A recombinant vector comprising:
- a) an isolated nucleic acid segment encoding a polyhydroxyalkanoic acid synthase protein:
- b) an isolated nucleic acid segment encoding a fatty acid:acyl-CoA transferase protein;
- and
- c) promoter heterologous to the nucleic acid segment encoding a polyhydroxyalkanoic acid synthase protein, and heterologous to the nucleic acid segment encoding a fatty acid:acyl-CoA transferase protein.
1 more element
A cell comprising an isolated nucleic acid segment, the nucleic acid segment encoding:
- a) a polyhydroxyalkanoic acid synthase protein;
- and
- b) a fatty acid:acyl-CoA transferase protein.
A method for preparing a transformed cell, comprising the steps:
- a) selecting a host cell;
- b) contacting the host cell and an isolated nucleic acid segment, the nucleic acid segment encoding:
- i) a polyhydroxyalkanoic acid synthase protein;
- and
- ii) a fatty acid:acyl-CoA transferase protein;
- under conditions suitable for uptake of the nucleic acid segment by the host cell;
- and
- c) regenerating the cell to produce a transformed cell;
- wherein the nucleic acid segment is free of total genomic DNA.
6 more elements
A method for the preparation of a polyester, comprising the steps of:
- a) obtaining a cell capable of producing:
- i) a polyhydroxyalkanoic acid synthase protein;
- and
- ii) a fatty acid:acyl-CoA transferase protein;
- b) establishing a culture of the cell;
- c) culturing the cell under conditions suitable for the production of the polyester;
- and
- d) isolating the polyester from the cell.
5 more elements
Dependencies are inferred from the claim text.