D-enzyme compositions and methods of their use
AssigneeThe Scripps Research Institute
InventorsStephen Brian Henry Kent, Saskia Charlotte Florence Milton, Raymond Cecil deLisle Milton
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Abstract
D-enzyme compositions are described comprising an amino acid residue sequence that defines an polypeptide able to catalyze an enzymatic reaction. The D-enzyme has an amino acid residue sequence consisting essentially of D-amino acids
Claims17
A D-enzyme comprising an amino acid residue sequence that defines an polypeptide able to catalyze an enzymatic reaction, said amino acid residue sequence consisting essentially of D-amino acids.
A method of producing a chirally pure chemical comprising:
- a) reacting in an aqueous admixture a first stereoisomer with a D-enzyme that specifically convert said first stereoisomer into a chiral reaction product, said reacting occurring for a time period and under reaction conditions sufficient to form said reaction product, wherein said D-enzyme comprises an amino acid residue sequence that defines an enzyme able to catalyze said reaction, and said amino acid residue sequence consists essentially of D-amino acids;
- and
- b) isolating said chiral reaction product from said admixture, thereby forming said chirally pure chemical.
A method of producing a chirally pure chemical comprising:
- a) reacting in an aqueous admixture a racemic mixture having at least a first and a second stereoisomer with a D-enzyme that specifically converts said first stereoisomer into a reaction product, said reacting occurring for a time period and under reaction conditions sufficient to convert substantially all of said first stereoisomer into said reaction product, wherein said D-enzyme comprises an amino acid residue sequence that defines an enzyme able to catalyze said reaction, and said amino acid residue sequence consists essentially of D-amino acids;
- and
- b) isolating said second stereoisomer from said admixture, thereby forming said chirally pure chemical.
A method for catalyzing a reaction involving an achiral substrate comprising the following steps:
- Step A:
- providing a D-enzyme having a specificity for the achiral substrate;
- then
- Step B:
- contacting the achiral substrate with the D-enzyme of said Step A to catalyze the reaction involving the achiral substrate.
A crystal comprising co-crystalized D- and L-enantiomers of an enzyme.
A method of structurally analyzing an L-enzyme comprising the following steps:
- Step A:
- providing a crystal comprising co-crystalized D- and L-enantiomers of the L-enzyme;
- then
- Step B:
- exposing the crystal of said Step A to X-rays for generating an X-ray pattern;
- and then
- Step C:
- analyzing the X-ray pattern generated in said Step B for structural information relating to the L-enzyme.
A method for screening a library for drug candidates having activity with respect to an L-enzyme, the method comprising the following steps:
- Step A:
- providing a D-enzyme, the D-enzyme being an enantiomer of the L-enzyme;
- then
- Step B:
- screening the library to identify one or more elements of the library having activity in connection with the D-enzyme;
- and then
- Step C:
- identifying drug candidates with respect to the L-enzyme using one or more of the elements of the library identified in said Step B.
A ligated D-protein represented by the formula:
- R—L—R′
- wherein R and R′ are D-peptides and L represents a linkage unit for ligating R to R′.
Dependencies are inferred from the claim text.