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OwnerMike Farwick, Klaus Huthmacher +3inventors
InventorsMike Farwick, Klaus Huthmacher, Achim Marx, Brigitte Bathe, Walter Pfefferle
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The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.
An isolated polynucleotide from coryneform bacteria, containing a polynucleotide sequence coding for the citE gene, selected from the group
A coryneform bacterium in which the citE gene is attenuated, especially excluded.
A process for the production of L-amino acids, especially L-lysine, by fermentation, which process comprises carrying out the following steps:
An isolated microorganism of the E. coli strain Top10/pCR2.1citEint, deposited at the Deutsche Sammlung fur Mikroorganismen und Zellkulturen in Braunschweig under number DSM 13981 in accordance with the Budapest Treaty.
The process as claimed in one or more of the preceding claims, wherein microorganisms of the species Corynebacterium glutamicum are used.
Dependencies are inferred from the claim text.