Polypeptides homologous to VEGF and BMP1
AssigneeGenentech, Inc.
InventorsNapoleone Ferrara, Sophia S. Kuo
Life of this patent
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Abstract
The present invention involves the identification and preparation of vascular endothelial growth factor-E (VEGF-E). VEGF-E is a novel polypeptide related to vascular endothelial growth factor (VEGF) and bone morphogenetic protein 1. VEGF-E has homology to VEGF including conservation of the amino acids required for activity of VEGF. VEGF-E can be useful in wound repair, as well as in the generation and regeneration of tissue.
Claims33
An isolated nucleic acid comprising a nucleotide sequence encoding a vascular endothelial cell growth factor-E (VEGF-E) polypeptide comprising amino acid residues 1 through 345 of FIG. 2 (SEQ ID NO:2).
A VEGF-E polypeptide comprising amino acid residues 1 through 345 of FIG. 2 (SEQ ID NO:2).
A VEGF-E polypeptide selected from the group consisting of:
- (a) a polypeptide comprising amino acid residues 1 through 345 of FIG. 2 (SEQ ID NO:2);
- and
- (b) a polypeptide fragment of (a), wherein said fragment is biologically active.
A VEGF-E polypeptide encoded by the nucleotide sequence insert of ATCC deposit No. 209653.
A method for diagnosing a disease or a susceptibility to a disease related to a mutation in a nucleic acid sequence encoding vascular endothelial cell growth factor-E (VEGF-E) comprising:
- (a) isolating a nucleic acid sequence encoding VEGF-E from a sample derived from a host;
- and
- (b) determining a mutation in the nucleic acid sequence encoding VEGF-E.
A method of diagnosing cardiovascular and endothelial disorders in a mammal comprising detecting the level of expression of a gene encoding a vascular endothelial cell growth factor-E (VEGF-E) polypeptide (a) in a test sample of tissue cells obtained from the mammal, and (b) in a control sample of known normal tissue cells of the same cell type, wherein a higher or lower expression level in the test sample indicates the presence of a cardiovascular or endothelial dysfunction in the mammal from which the test tissue cells were obtained.
A method for identifying an agonist to a vascular endothelial cell growth factor-E (VEGF-E) polypeptide comprising:
- (a) contacting cells and a candidate compound under conditions that allow the polypeptide to stimulate proliferation of the cells;
- and
- (b) measuring the extent to which cell proliferation is inhibited by the compound.
A method for identifying a compound that inhibits the expression or activity of a vascular endothelial cell growth factor-E (VEGF-E) polypeptide, comprising:
- (a) contacting a candidate compound with the polypeptide under conditions and for a time sufficient to allow the compound and polypeptide to interact;
- and
- (b) measuring the extent to which the compound interacts with the polypeptide.
An isolated antibody that binds a vascular endothelial cell growth factor-E (VEGF-E) polypeptide.
Dependencies are inferred from the claim text.