Method for reconstituting a recombinant protein to its biologically active form
AssigneeBoehringer Ingelheim Austria GmbH
InventorsRoman Necina, Robert Schlegl, Alois Jungbauer, Christine Machold
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Abstract
In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.
Claims6
A method for obtaining a biologically active recombinant protein by reconstituting the protein from a denatured state to its active form, wherein a feed solution containing the recombinant protein of interest in its denatured form and/or in biologically inactive intermediate forms is subject to a chromatographic separation process, in which said protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein, characterized in that the denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous chromatographic method.
Dependencies are inferred from the claim text.